Comprehensive evaluation of the antioxidant capacity of Sceptridium ternatum using multiple colorimetric methods and 1,1-diphenyl-2-picrylhydrazyl-high-performance liquid chromatography analysis.
antioxidants
colorimetric methods
herbal medicine
mass spectrometry
Journal
Journal of separation science
ISSN: 1615-9314
Titre abrégé: J Sep Sci
Pays: Germany
ID NLM: 101088554
Informations de publication
Date de publication:
Sep 2020
Sep 2020
Historique:
received:
16
05
2020
revised:
05
07
2020
accepted:
19
07
2020
pubmed:
23
7
2020
medline:
31
8
2021
entrez:
23
7
2020
Statut:
ppublish
Résumé
Sceptridium ternatum is a medicinal herb with multiple health benefits. However, its antioxidant activity and active components have not been clarified. In this study, the antioxidant capacity of S. ternatum was comprehensively investigated using multiple colorimetric methods and 1,1-diphenyl-2-picrylhydrazyl-high-performance liquid chromatography analysis. First, the phenolic content, flavonoid content, and radical scavenging ability of S. ternatum were parallelly determined using colorimetric methods performed in 96-well microplates. The flavonoid content, rather than the phenolic content, was highly correlated with its antioxidant activity. Sceptridium ternatum was shown to be a rich source of flavonoids, with a highest flavonoid yield of 3.44 ± 0.11 mg/g. Subsequently, 1,1-diphenyl-2-picrylhydrazyl-high-performance liquid chromatography experiment and quadrupole time-of-flight mass spectrometry analyses were carried out for rapid screening of the individual antioxidants. A total of 14 O-glycosyl flavonoids with quercetin or kaempferol aglycone have been characterized. Particularly, quercetin 3-O-rhamnoside-7-O-glucoside exhibited the most potent antioxidant ability. Its half-maximal effective concentrations for scavenging 1,1-diphenyl-2-picrylhydrazyl and 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) radicals were 70.55 ± 2.69 and 106.90 ± 1.76 µg/mL, respectively, which were comparable with those of l-ascorbic acid. Our results indicated that the combined colorimetric and chromatographic methods provided a practical strategy for the discovery of bioactive compounds from natural products.
Identifiants
pubmed: 32697390
doi: 10.1002/jssc.202000550
doi:
Substances chimiques
Antioxidants
0
Biphenyl Compounds
0
Picrates
0
1,1-diphenyl-2-picrylhydrazyl
DFD3H4VGDH
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3615-3624Subventions
Organisme : Zhejiang Provincial Natural Science Foundation of China
ID : LQ19H280002
Organisme : Zhejiang Provincial Natural Science Foundation of China
ID : YY18H310023
Organisme : Hospital Pharmacy Research Project from Zhejiang Pharmaceutical Association
ID : 2018ZYY08
Organisme : Projects of Medical and Health Technology Program in Zhejiang Province
ID : 2018KY286
Organisme : Projects of Medical and Health Technology Program in Zhejiang Province
ID : 2017KY244
Organisme : Projects of Medical and Health Technology Program in Zhejiang Province
ID : 2016KYB033
Organisme : Projects of Medical and Health Technology Program in Zhejiang Province
ID : 2015KYB061
Informations de copyright
© 2020 Wiley-VCH GmbH.
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