Fast, Simultaneous Tagging and Mutagenesis of Genes on Bacterial Chromosomes.

Chromosomes, Bacterial / genetics Escherichia coli / genetics Escherichia coli Proteins / genetics Luminescent Proteins / genetics Monosaccharide Transport Proteins / genetics Mutagenesis Plasmids / genetics Symporters / genetics Red Fluorescent Protein
fluorescence microscopy genome engineering point mutagenesis protein tagging recombineering

Journal

ACS synthetic biology
ISSN: 2161-5063
Titre abrégé: ACS Synth Biol
Pays: United States
ID NLM: 101575075

Informations de publication

Date de publication:
21 08 2020
Historique:
pubmed: 10 7 2020
medline: 28 5 2021
entrez: 10 7 2020
Statut: ppublish

Résumé

Fluorescence microscopy has become a powerful tool in molecular cell biology. Visualizing specific proteins in bacterial cells requires labeling with fluorescent or fluorogenic tags, preferentially at the native chromosomal locus to preserve expression dynamics associated with the genomic environment. Exploring protein function calls for targeted mutagenesis and observation of differential phenotypes. In the model bacterium

Identifiants

DOI: 10.1021/acssynbio.0c00202 PMID: 32645263
pubmed: 32645263
doi: 10.1021/acssynbio.0c00202
doi:

Substances chimiques

Escherichia coli Proteins 0
LacY protein, E coli 0
Luminescent Proteins 0
Monosaccharide Transport Proteins 0
Symporters 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

2203-2207

Auteurs

Leonard Schärfen (L)

B CUBE, TU Dresden, Tatzberg 41, 01307 Dresden, Germany.
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, United States.
ORCID: 0000-0003-0234-7609

Miloš Tišma (M)

B CUBE, TU Dresden, Tatzberg 41, 01307 Dresden, Germany.
Department of Bionanoscience, Delft University of Technology, Delft 2629HZ, Netherlands.

Michael Schlierf (M)

B CUBE, TU Dresden, Tatzberg 41, 01307 Dresden, Germany.
Cluster of Excellence Physics of Life, TU Dresden, 01062 Dresden, Germany.
ORCID: 0000-0002-6209-2364

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Classifications MeSH

Recherchemedicale.com › Phénomènes génétiques › Structures génétiques › Chromosomes › Chromosomes de bactérie › Fast, Simultaneous Tagging and Mutagenesis of...
Recherchemedicale.com › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › Fast, Simultaneous Tagging and Mutagenesis of...
Recherchemedicale.com › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Protéines Escherichia coli › Fast, Simultaneous Tagging and Mutagenesis of...
Recherchemedicale.com › Acides aminés, peptides et protéines › Protéines › Protéines luminescentes › Fast, Simultaneous Tagging and Mutagenesis of...
Recherchemedicale.com › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de transport membranaire › Transporteurs de monosaccharides › Fast, Simultaneous Tagging and Mutagenesis of...
Recherchemedicale.com › Phénomènes génétiques › Mutagenèse › Fast, Simultaneous Tagging and Mutagenesis of...
Recherchemedicale.com › Phénomènes génétiques › Structures génétiques › Plasmides › Fast, Simultaneous Tagging and Mutagenesis of...
Recherchemedicale.com › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de transport membranaire › Pompes ioniques › Symporteurs › Fast, Simultaneous Tagging and Mutagenesis of...