Off-Peak 594-nm Light Surpasses On-Peak 532-nm Light in Silencing Distant ArchT-Expressing Neurons In Vivo.
Neuroscience
Optical Imaging
Technical Aspects of Cell Biology
Journal
iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038
Informations de publication
Date de publication:
24 Jul 2020
24 Jul 2020
Historique:
received:
04
12
2019
revised:
23
03
2020
accepted:
11
06
2020
pubmed:
1
7
2020
medline:
1
7
2020
entrez:
30
6
2020
Statut:
ppublish
Résumé
For large brain volume manipulations using optogenetics, both effective opsin excitation and efficient light delivery with minimal light absorption are required to minimize the illuminating light intensity and concomitant off-target effects. ArchT, a widely used potent inhibitory opsin, is commonly activated by 532-nm light, which lies on its in vitro excitation peak. However, 532-nm light also lies on a peak range of the hemoglobin absorption spectrum. Therefore, we predicted that 594-nm light is superior in suppressing distant ArchT-expressing neurons, which is slightly off the ArchT-excitation-plateau and largely off the peak of the hemoglobin absorption spectrum. We quantitatively tested this prediction by the electrophysiological recording of the rat cortex in vivo. At illumination distances greater than 500 μm, 594-nm light was more effective than 532-nm light. Its superiority increased with distance. These results validate our prediction and highlight the significance of excitation-absorption trade-off in selecting illumination wavelength for optogenetics in vivo.
Identifiants
pubmed: 32599561
pii: S2589-0042(20)30462-4
doi: 10.1016/j.isci.2020.101276
pmc: PMC7326739
pii:
doi:
Types de publication
Journal Article
Langues
eng
Pagination
101276Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing financial interests.
Références
Neuron. 2017 Aug 2;95(3):504-529
pubmed: 28772120
J Neurosci Methods. 2012 Oct 15;211(1):49-57
pubmed: 22971353
Nature. 2011 Mar 17;471(7338):358-62
pubmed: 21389985
Neuron. 2018 Feb 21;97(4):980-989.e6
pubmed: 29395916
Nat Neurosci. 2014 Aug;17(8):1123-9
pubmed: 24997763
Neurosurgery. 1987 Oct;21(4):459-64
pubmed: 3683777
Nat Neurosci. 2019 Jul;22(7):1061-1065
pubmed: 31209378
Front Neurosci. 2018 Jul 05;12:464
pubmed: 30026686
Biomed Opt Express. 2016 Oct 06;7(11):4450-4471
pubmed: 27895987
Nat Neurosci. 2016 Apr;19(4):564-7
pubmed: 26900924
Cell. 2016 Nov 3;167(4):961-972.e16
pubmed: 27773481
J Neurosci. 2013 Oct 16;33(42):16684-97
pubmed: 24133271
J Biophotonics. 2016 Jun;9(6):576-85
pubmed: 26192551
J Neural Eng. 2007 Sep;4(3):S143-56
pubmed: 17873414
PLoS Biol. 2015 Feb 27;13(2):e1002070
pubmed: 25723479
Science. 2017 Aug 18;357(6352):687-692
pubmed: 28818944
Nature. 2014 Nov 13;515(7526):269-73
pubmed: 25383519
Elife. 2019 Nov 18;8:
pubmed: 31736463
Science. 2015 Aug 7;349(6248):647-50
pubmed: 26113638
Phys Med Biol. 2002 Jun 21;47(12):2059-73
pubmed: 12118601
Nat Neurosci. 2009 Feb;12(2):229-34
pubmed: 19079251
Nat Rev Neurosci. 2012 Mar 20;13(4):251-66
pubmed: 22430017
Neuron. 2011 Jul 14;71(1):9-34
pubmed: 21745635
Mol Psychiatry. 2018 Apr;23(4):914-922
pubmed: 28439099
Opt Lett. 2015 May 15;40(10):2173-6
pubmed: 26393692
Front Neurosci. 2018 Oct 26;12:771
pubmed: 30416424
Nat Methods. 2014 Mar;11(3):338-46
pubmed: 24509633
Neurophotonics. 2016 Oct;3(4):045007
pubmed: 27981063
Science. 2009 Apr 17;324(5925):354-9
pubmed: 19299587
J Biomed Opt. 2010 Sep-Oct;15(5):057005
pubmed: 21054121
J Neurosci. 2013 Aug 21;33(34):13654-62
pubmed: 23966687
Biomed Opt Express. 2010 Jul 27;1(1):310-317
pubmed: 21258468
Front Syst Neurosci. 2011 Apr 13;5:18
pubmed: 21811444
Curr Biol. 2015 Oct 19;25(20):2642-50
pubmed: 26441351
Philos Trans R Soc Lond B Biol Sci. 1996 Oct 29;351(1346):1445-53
pubmed: 8941956
Neuron. 2012 Dec 6;76(5):901-7
pubmed: 23217739
Nature. 2008 Jan 3;451(7174):61-4
pubmed: 18094685
Nat Commun. 2019 Apr 30;10(1):1993
pubmed: 31040285
Biochemistry. 2010 Jan 19;49(2):267-78
pubmed: 20000562
J Neurosci. 2017 Apr 26;37(17):4565-4583
pubmed: 28363984
Proc Natl Acad Sci U S A. 2015 May 26;112(21):6730-5
pubmed: 25953336
J Neurosci. 2017 Nov 8;37(45):10894-10903
pubmed: 29118219
Nat Commun. 2018 Oct 8;9(1):4125
pubmed: 30297821
J Neurosci. 2014 May 14;34(20):6896-909
pubmed: 24828644
Nat Neurosci. 2017 Aug;20(8):1180-1188
pubmed: 28628101
Nat Rev Neurosci. 2017 Mar 17;18(4):222-235
pubmed: 28303019
Cell Rep. 2015 Jul 21;12(3):525-34
pubmed: 26166563
Nat Methods. 2011 Dec 18;9(2):159-72
pubmed: 22179551
IEEE Trans Biomed Eng. 1979 Dec;26(12):656-64
pubmed: 544437
Annu Rev Neurosci. 2004;27:279-306
pubmed: 15217334
Science. 2017 Jan 13;355(6321):188-193
pubmed: 28082592
Proc Natl Acad Sci U S A. 2016 Nov 15;113(46):E7297-E7306
pubmed: 27807140
Nat Commun. 2017 Jan 31;8:14191
pubmed: 28139643