HERQ-9 Is a New Multiplex PCR for Differentiation and Quantification of All Nine Human Herpesviruses.
Adolescent
Adult
Aged
Cell Line
Child
Child, Preschool
Computer Simulation
DNA Primers
/ genetics
DNA Probes
/ genetics
DNA, Viral
/ genetics
Herpesviridae
/ classification
Herpesviridae Infections
/ diagnosis
Humans
Middle Aged
Multiplex Polymerase Chain Reaction
/ methods
Palatine Tonsil
/ virology
Reproducibility of Results
Sensitivity and Specificity
Young Adult
Epstein-Barr virus
HHV-6
coinfection
cytomegalovirus
diagnostics
human herpesviruses
multiplex
qPCR
quantitative methods
tonsils
viral persistence
virome
Journal
mSphere
ISSN: 2379-5042
Titre abrégé: mSphere
Pays: United States
ID NLM: 101674533
Informations de publication
Date de publication:
24 06 2020
24 06 2020
Historique:
entrez:
26
6
2020
pubmed:
26
6
2020
medline:
16
7
2021
Statut:
epublish
Résumé
Infections with the nine human herpesviruses (HHVs) are globally prevalent and characterized by lifelong persistence. Reactivations can potentially manifest as life-threatening conditions for which the demonstration of viral DNA is essential. In the present study, we developed HERQ-9, a pan-HHV quantitative PCR designed in triplex reactions to differentiate and quantify each of the HHV-DNAs: (i) herpes simplex viruses 1 and 2 and varicella-zoster virus; (ii) Epstein-Barr virus, human cytomegalovirus, and Kaposi's sarcoma-associated herpesvirus; and (iii) HHV-6A, -6B, and -7. The method was validated with prequantified reference standards as well as with mucocutaneous swabs and cerebrospinal fluid, plasma, and tonsillar tissue samples. Our findings highlight the value of multiplexing in the diagnosis of many unsuspected, yet clinically relevant, herpesviruses. In addition, we report here frequent HHV-DNA co-occurrences in clinical samples, including some previously unknown. HERQ-9 exhibited high specificity and sensitivity (LOD
Identifiants
pubmed: 32581076
pii: 5/3/e00265-20
doi: 10.1128/mSphere.00265-20
pmc: PMC7316487
pii:
doi:
Substances chimiques
DNA Primers
0
DNA Probes
0
DNA, Viral
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2020 Pyöriä et al.
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