In vivo kinetics of the genotoxic and cytotoxic activities of cladribine and clofarabine.
cytometry
cytotoxicokinetics
genotoxicokinetics
micronucleus assay
normoblasts
Journal
Environmental and molecular mutagenesis
ISSN: 1098-2280
Titre abrégé: Environ Mol Mutagen
Pays: United States
ID NLM: 8800109
Informations de publication
Date de publication:
11 2020
11 2020
Historique:
received:
23
03
2020
revised:
01
05
2020
accepted:
10
05
2020
pubmed:
23
6
2020
medline:
9
2
2021
entrez:
23
6
2020
Statut:
ppublish
Résumé
The aim of the present in vivo study was to determine the kinetics of the genotoxic and cytotoxic activities of cladribine and clofarabine in mouse normoblasts using flow cytometry. Mice in groups of five were treated with cladribine or clofarabine. Blood samples were obtained from the mouse tails before treatment and every 8 hr posttreatment for 72 hr. These samples were cytometrically scored for micronucleated reticulocytes (RETs), and the percentage of RETs was determined. The results showed that clofarabine and cladribine have early cytotoxic effects that are related to the genotoxic effects reported in previous studies; the drugs have both complex long-lasting genotoxic and cytotoxic kinetic activity, with similar profiles that suggest a relationship between the genotoxic and cytotoxic parameters. The initial genotoxkinetics timing of clofarabine is equivalent to those of difluorodeoxycytidine, likely because both agents inhibit DNA polymerase. Clofarabine shows a higher genotoxic and cytotoxic efficiency than cladribine, in agreement with previous results.
Substances chimiques
Antineoplastic Agents
0
Cladribine
47M74X9YT5
Clofarabine
762RDY0Y2H
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
922-927Subventions
Organisme : Consejo Nacional de Ciencia y Tecnología
ID : CB-240116-2014
Pays : International
Informations de copyright
© 2020 Wiley Periodicals LLC.
Références
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