Mitochondrial protein biogenesis in the synapse is supported by local translation.
fragile X syndrome
mitochondria
synapses
translation
Journal
EMBO reports
ISSN: 1469-3178
Titre abrégé: EMBO Rep
Pays: England
ID NLM: 100963049
Informations de publication
Date de publication:
05 08 2020
05 08 2020
Historique:
received:
30
09
2019
revised:
21
04
2020
accepted:
15
05
2020
pubmed:
20
6
2020
medline:
28
4
2021
entrez:
20
6
2020
Statut:
ppublish
Résumé
Synapses are the regions of the neuron that enable the transmission and propagation of action potentials on the cost of high energy consumption and elevated demand for mitochondrial ATP production. The rapid changes in local energetic requirements at dendritic spines imply the role of mitochondria in the maintenance of their homeostasis. Using global proteomic analysis supported with complementary experimental approaches, we show that an essential pool of mitochondrial proteins is locally produced at the synapse indicating that mitochondrial protein biogenesis takes place locally to maintain functional mitochondria in axons and dendrites. Furthermore, we show that stimulation of synaptoneurosomes induces the local synthesis of mitochondrial proteins that are transported to the mitochondria and incorporated into the protein supercomplexes of the respiratory chain. Importantly, in a mouse model of fragile X syndrome, Fmr1 KO mice, a common disease associated with dysregulation of synaptic protein synthesis, we observed altered morphology and respiration rates of synaptic mitochondria. That indicates that the local production of mitochondrial proteins plays an essential role in synaptic functions.
Identifiants
pubmed: 32558077
doi: 10.15252/embr.201948882
pmc: PMC7403725
doi:
Substances chimiques
Fmr1 protein, mouse
0
Mitochondrial Proteins
0
Fragile X Mental Retardation Protein
139135-51-6
Banques de données
GEO
['GSE122724']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e48882Subventions
Organisme : Narodowe Centrum Nauki (NCN)
ID : SONATA BIS 2014/14/E/NZ3/00375
Pays : International
Organisme : Narodowe Centrum Nauki (NCN)
ID : OPUS6 2013/11/B/NZ3/00974
Pays : International
Organisme : Narodowe Centrum Nauki (NCN)
ID : OPUS10 2015/19/B/NZ3/03272
Pays : International
Organisme : Fundacja na rzecz Nauki Polskiej (FNP)
ID : MAB/2017/2
Pays : International
Informations de copyright
© 2020 The Authors. Published under the terms of the CC BY 4.0 license.
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