Draft genome sequence of antimicrobial producing Paenibacillus alvei strain MP1 reveals putative novel antimicrobials.

Antimicrobial-producing strain NRPS Paenibacillus alvei Whole genome sequencing

Journal

BMC research notes
ISSN: 1756-0500
Titre abrégé: BMC Res Notes
Pays: England
ID NLM: 101462768

Informations de publication

Date de publication:
09 Jun 2020
Historique:
received: 16 02 2020
accepted: 04 06 2020
entrez: 11 6 2020
pubmed: 11 6 2020
medline: 6 3 2021
Statut: epublish

Résumé

A Paenibacillus strain isolated in previous research exhibited antimicrobial activity against relevant human pathogens including Staphylococcus aureus and Listeria monocytogenes. In this study, the genome of the aforementioned strain, designated as MP1, was shotgun sequenced. The draft genome of strain MP1 was subject to multiple genomic analyses to taxonomically characterize it and identify the genes potentially responsible for its antimicrobial activity. Here we report the draft genome sequence of an antimicrobial producing Paenibacillus strain, MP1. Average Nucleotide Identity (ANI) analysis established strain MP1 as a new strain of the previously characterized Paenibacillus alvei. The genomic analysis identified several putative secondary metabolite clusters including seven Nonribosomal Peptide Synthetase clusters (NRPS) (> 10,000 nt), one bacteriocin or other unspecified Ribosomally Synthesized and Post-Translationally modified Peptide Product (RiPP), one lanthipeptide, and six hybrid clusters (NRPS-Type I Polyketide synthase (T1PKS) and NRPS-trans Amino Transferase Polyketide Synthase (AT-PKS)).

Identifiants

pubmed: 32517793
doi: 10.1186/s13104-020-05124-z
pii: 10.1186/s13104-020-05124-z
pmc: PMC7285544
doi:

Substances chimiques

Anti-Infective Agents 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

280

Subventions

Organisme : Narodowe Centrum Nauki
ID : 2015/18/E/NZ6/00700
Organisme : College of Agriculture and Life Sciences, Cornell University
ID : S1077 Multi-state grant

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Auteurs

Magdalena Pajor (M)

Department of Pharmaceutical Technology and Biochemistry, Faculty of Chemistry, Gdansk University of Technology, G. Narutowicza Street 11/12, 80-233, Gdańsk, Poland. magdalena.pajor@pg.edu.pl.

Jonathan Sogin (J)

Department of Food Science, Cornell University, Stocking Hall, Ithaca, NY, 14853-7201, USA.

Randy W Worobo (RW)

Department of Food Science, Cornell University, Stocking Hall, Ithaca, NY, 14853-7201, USA.

Piotr Szweda (P)

Department of Pharmaceutical Technology and Biochemistry, Faculty of Chemistry, Gdansk University of Technology, G. Narutowicza Street 11/12, 80-233, Gdańsk, Poland.

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Classifications MeSH