Identification of genomic regions associated with shoot fly resistance in maize and their syntenic relationships in the sorghum genome.
Animals
Chromosome Mapping
/ methods
Disease Resistance
/ genetics
Edible Grain
/ genetics
Genomics
/ methods
Genotype
India
Insecta
Microsatellite Repeats
/ genetics
Pest Control, Biological
/ methods
Phenotype
Plant Diseases
/ genetics
Quantitative Trait Loci
/ genetics
Sorghum
/ genetics
Zea mays
/ genetics
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
30
03
2020
accepted:
22
05
2020
entrez:
10
6
2020
pubmed:
10
6
2020
medline:
26
8
2020
Statut:
epublish
Résumé
Shoot fly (Atherigona naqvii) is one of the major insects affecting spring maize in North India and can cause yield loss up to 60 per cent. The genetics of insect resistance is complex as influenced by genotypic background, insect population and climatic conditions. Therefore, quantitative trait loci (QTL) mapping is a highly effective approach for studying genetically complex forms of insect resistance. The objective of the present study was to dissect the genetic basis of resistance and identification of genomic regions associated with shoot fly resistance. A total of 107 F2 population derived from the cross CM143 (resistant) x CM144 (susceptible) was genotyped with 120 SSR markers. Phenotypic data were recorded on replicated F2:3 progenies for various component traits imparting resistance to shoot fly at different time intervals. Resistance to shoot fly was observed to be under polygenic control as evidenced by the identification of 19 putative QTLs governed by overdominance to partial dominance and additive gene actions. The major QTLs conditioning shoot fly resistance viz., qDH9.1 (deadheart) and qEC9.1 (oviposition) explaining 15.03 and 18.89 per cent phenotypic variance, respectively were colocalized on chromosome 9. These QTLs are syntenic to regions of chromosome 10 of sorghum which were also accounted for deadheart and oviposition suggesting that the same gene block may be responsible for shoot fly resistance. The candidate genes such as cysteine protease, subtilisin-chymotrypsin inhibitor, cytochrome P450 involved in synthesis of alleochemicals, receptor kinases, glossy15 and ubiquitin-proteasome degradation pathway were identified within the predicted QTL regions. This is the first reported mapping of QTLs conferring resistance to shoot fly in maize, and the markers identified here will be a valuable resource for developing elite maize cultivars with resistance to shoot fly.
Identifiants
pubmed: 32516348
doi: 10.1371/journal.pone.0234335
pii: PONE-D-20-09060
pmc: PMC7282634
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0234335Déclaration de conflit d'intérêts
The authors declare that they have no conflict of interest.
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