Evolving a Thermostable Terminal Deoxynucleotidyl Transferase.

Amino Acid Sequence Animals Catalysis Cattle DNA Nucleotidylexotransferase / chemistry DNA, Single-Stranded / chemistry DNA-Directed DNA Polymerase / chemistry Escherichia coli / genetics Kinetics Mutagenesis Mutant Proteins / chemistry Oligonucleotides / chemistry Plasmids / genetics Protein Engineering / methods Temperature
TdT modified nucleotides protein engineering terminal deoxynucleotidyl transferase thermostability thermostable TdT

Journal

ACS synthetic biology
ISSN: 2161-5063
Titre abrégé: ACS Synth Biol
Pays: United States
ID NLM: 101575075

Informations de publication

Date de publication:
17 07 2020
Historique:
pubmed: 5 6 2020
medline: 20 7 2021
entrez: 5 6 2020
Statut: ppublish

Résumé

Terminal deoxynucleotidyl transferase (TdT) catalyzes template free incorporation of arbitrary nucleotides onto single-stranded DNA. Due to this unique feature, TdT is widely used in biotechnology and clinical applications. One particularly tantalizing use is the synthesis of long

Identifiants

DOI: 10.1021/acssynbio.0c00078 PMID: 32497424
pubmed: 32497424
doi: 10.1021/acssynbio.0c00078
doi:

Substances chimiques

DNA, Single-Stranded 0
Mutant Proteins 0
Oligonucleotides 0
DNA Nucleotidylexotransferase EC 2.7.7.31
DNA-Directed DNA Polymerase EC 2.7.7.7

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1725-1735

Auteurs

Jasmine Puay Suan Chua (JPS)

Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 8 Medical Drive, Singapore 117597.
NUS Synthetic Biology for Clinical and Technological Innovation, 14 Medical Drive, Singapore 117599.
Illumina Singapore Pte, Ltd., 29 Woodlands Industrial Park E1, North Tech Building, Singapore 757716.
ORCID: 0000-0002-4388-0648

Maybelle Kho Go (MK)

Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 8 Medical Drive, Singapore 117597.
NUS Synthetic Biology for Clinical and Technological Innovation, 14 Medical Drive, Singapore 117599.

Trina Osothprarop (T)

Illumina Inc., 5200 Illumina Way, San Diego, California 92122, United States.

Seth Mcdonald (S)

Illumina Inc., 5200 Illumina Way, San Diego, California 92122, United States.

Alexander G Karabadzhak (AG)

Illumina Inc., 5200 Illumina Way, San Diego, California 92122, United States.

Wen Shan Yew (WS)

Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 8 Medical Drive, Singapore 117597.
NUS Synthetic Biology for Clinical and Technological Innovation, 14 Medical Drive, Singapore 117599.
ORCID: 0000-0002-3021-0469

Sergio Peisajovich (S)

Illumina Inc., 5200 Illumina Way, San Diego, California 92122, United States.

Saurabh Nirantar (S)

Illumina Singapore Pte, Ltd., 29 Woodlands Industrial Park E1, North Tech Building, Singapore 757716.

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Classifications MeSH

Recherchemedicale.com › Sciences de l'information › Sources d'information › Services d'information › Documentation › Données de séquences moléculaires › Séquence d'acides aminés › Evolving a Thermostable Terminal...
Recherchemedicale.com › Eucaryotes › Animaux › Evolving a Thermostable Terminal...
Recherchemedicale.com › Phénomènes chimiques › Catalyse › Evolving a Thermostable Terminal...
Recherchemedicale.com › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Artiodactyla › Ruminants › Bovins › Evolving a Thermostable Terminal...
Recherchemedicale.com › Enzymes et coenzymes › Enzymes › Transferases › Phosphotransferases › Nucleotidyltransferases › DNA nucleotidyltransferases › DNA nucleotidylexotransferase › Evolving a Thermostable Terminal...
Recherchemedicale.com › Phénomènes génétiques › Structures génétiques › Conformation d'acide nucléique › ADN simple brin › Evolving a Thermostable Terminal...
Recherchemedicale.com › Enzymes et coenzymes › Enzymes › Transferases › Phosphotransferases › Nucleotidyltransferases › DNA nucleotidyltransferases › DNA-directed DNA polymerase › Evolving a Thermostable Terminal...
Recherchemedicale.com › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › Evolving a Thermostable Terminal...
Recherchemedicale.com › Phénomènes chimiques › Phénomènes biochimiques › Cinétique › Evolving a Thermostable Terminal...
Recherchemedicale.com › Phénomènes génétiques › Mutagenèse › Evolving a Thermostable Terminal...
Recherchemedicale.com › Acides aminés, peptides et protéines › Protéines › Protéines mutantes › Evolving a Thermostable Terminal...
Recherchemedicale.com › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Polynucléotides › Oligonucléotides › Evolving a Thermostable Terminal...
Recherchemedicale.com › Phénomènes génétiques › Structures génétiques › Plasmides › Evolving a Thermostable Terminal...
Recherchemedicale.com › Techniques d'investigation › Techniques génétiques › Génie génétique › Ingénierie des protéines › Evolving a Thermostable Terminal...
Recherchemedicale.com › Environnement et santé publique › Environnement › Concepts météorologiques › Atmosphère › Temps (météorologie) › Température › Evolving a Thermostable Terminal...