Radioprotection of thymine and calf thymus DNA by an azo compound: mechanism of action followed by DPPH radical quenching & ROS depletion in WI 38 lung fibroblast cells.
2-(2-hydroxyphenylazo)-indole-3∕-acetic acid (HPIA)
Calf thymus DNA
DCFDA
DPPH
Pharmaceutical chemistry
Radioprotection
Thymine
Journal
Heliyon
ISSN: 2405-8440
Titre abrégé: Heliyon
Pays: England
ID NLM: 101672560
Informations de publication
Date de publication:
May 2020
May 2020
Historique:
received:
15
03
2020
revised:
26
04
2020
accepted:
18
05
2020
entrez:
4
6
2020
pubmed:
4
6
2020
medline:
4
6
2020
Statut:
epublish
Résumé
To explain the observed radio-protection properties of an azo compound, 2-(2-hydroxyphenylazo)-indole-3 Mechanism of radioprotection by HPIA was attempted using the stable free radical 2, 2-diphenyl-1-picrylhydrazyl (DPPH) using UV-Vis and electron paramagnetic resonance (EPR) spectroscopy. The radical destroying ability of HPIA was studied by depletion of reactive oxygen species (ROS) in WI 38 lung fibroblast cells. Studies indicate HPIA interacts with radical intermediates formed in solution following irradiation by The study indicates HPIA may be an antioxidant supplement during radiotherapy.
Identifiants
pubmed: 32490245
doi: 10.1016/j.heliyon.2020.e04036
pii: S2405-8440(20)30880-X
pii: e04036
pmc: PMC7262411
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e04036Informations de copyright
© 2020 The Author(s).
Références
Acta Oncol. 2003;42(5-6):357-65
pubmed: 14596499
J Org Chem. 2004 Apr 2;69(7):2309-14
pubmed: 15049623
Free Radic Biol Med. 1999 Sep;27(5-6):612-6
pubmed: 10490282
Mutat Res. 1983 Apr;114(3):269-81
pubmed: 6339890
Biochem Pharmacol. 2003 May 15;65(10):1575-82
pubmed: 12754093
Int J Radiat Oncol Biol Phys. 1982 Mar-Apr;8(3-4):565-70
pubmed: 7050040
J Environ Pathol Toxicol Oncol. 2012;31(2):155-66
pubmed: 23216640
Biochim Biophys Acta. 1999 Nov 16;1472(3):643-50
pubmed: 10564778
Radiat Environ Biophys. 1984;23(1):1-6
pubmed: 6709825
Proc Soc Exp Biol Med. 2000 Oct;225(1):9-22
pubmed: 10998194
J Agric Food Chem. 2015 Oct 14;63(40):8765-76
pubmed: 26390267
Int J Immunopharmacol. 2000 Jan;22(1):1-14
pubmed: 10684984
Oncologist. 2010;15(4):360-71
pubmed: 20413641
J Biochem Biophys Methods. 2005 Dec 31;65(2-3):45-80
pubmed: 16297980
Chem Biol Interact. 2011 Feb 1;189(3):206-14
pubmed: 21110954
Carcinogenesis. 2001 Aug;22(8):1189-93
pubmed: 11470748
Biol Pharm Bull. 2015;38(10):1478-83
pubmed: 26424013
Radiat Res. 1982 Nov;92(2):245-54
pubmed: 7163478
PLoS One. 2018 Apr 26;13(4):e0196310
pubmed: 29698534
Water Res. 2005 Apr;39(8):1425-40
pubmed: 15878014
J Nutr Biochem. 2002 Oct;13(10):572-584
pubmed: 12550068
Environ Health Perspect. 1997 Dec;105 Suppl 6:1459-62
pubmed: 9467063
Curr Protein Pept Sci. 2006 Apr;7(2):101-11
pubmed: 16611136
Radiat Phys Chem Oxf Engl 1993. 2015 Oct;115:127-134
pubmed: 29276348
Radiat Environ Biophys. 1990;29(4):249-62
pubmed: 2281132
Biochem Biophys Res Commun. 1974 Nov 27;61(2):396-403
pubmed: 4477007
Br J Radiol. 2010 Jul;83(991):554-68
pubmed: 20603408
J Agric Food Chem. 2014 May 14;62(19):4251-60
pubmed: 24738928
Free Radic Biol Med. 2012 Jan 1;52(1):1-6
pubmed: 22027063