Complex Trait Loci in Maize Enabled by CRISPR-Cas9 Mediated Gene Insertion.
CRISPR-Cas9
complex trait loci
gene expression
gene target
genetic crossing
maize
recombinase-mediated cassette exchange
trait stack
Journal
Frontiers in plant science
ISSN: 1664-462X
Titre abrégé: Front Plant Sci
Pays: Switzerland
ID NLM: 101568200
Informations de publication
Date de publication:
2020
2020
Historique:
received:
17
01
2020
accepted:
08
04
2020
entrez:
21
5
2020
pubmed:
21
5
2020
medline:
21
5
2020
Statut:
epublish
Résumé
Modern maize hybrids often contain biotech and native traits. To-date all biotech traits have been randomly inserted in the genome. Consequently, developing hybrids with multiple traits is expensive, time-consuming, and complex. Here we report using CRISPR-Cas9 to generate a complex trait locus (CTL) to facilitate trait stacking. A CTL consists of multiple preselected sites positioned within a small well-characterized chromosomal region where trait genes are inserted. We generated individual lines, each carrying a site-specific insertion landing pad (SSILP) that was targeted to a preselected site and capable of efficiently receiving a transgene via recombinase-mediated cassette exchange. The selected sites supported consistent transgene expression and the SSILP insertion had no effect on grain yield. We demonstrated that two traits residing at different sites within a CTL can be combined via genetic recombination. CTL technology is a major step forward in the development of multi-trait maize hybrids.
Identifiants
pubmed: 32431725
doi: 10.3389/fpls.2020.00535
pmc: PMC7214728
doi:
Types de publication
Journal Article
Langues
eng
Pagination
535Informations de copyright
Copyright © 2020 Gao, Mutti, Young, Yang, Schroder, Lenderts, Wang, Peterson, St. Clair, Jones, Feigenbutz, Marsh, Zeng, Wagner, Farrell, Snopek, Scelonge, Sopko, Sander, Betts, Cigan and Chilcoat.
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