Extracts of Thai Perilla frutescens nutlets attenuate tumour necrosis factor-α-activated generation of microparticles, ICAM-1 and IL-6 in human endothelial cells.
Atherosclerosis
/ drug therapy
Cell Line
Cell-Derived Microparticles
/ metabolism
Drug Evaluation, Preclinical
Endothelial Cells
/ drug effects
Endothelium, Vascular
/ cytology
Humans
Intercellular Adhesion Molecule-1
/ metabolism
Interleukin-6
/ metabolism
Nuts
/ chemistry
Perilla frutescens
/ chemistry
Plant Extracts
/ isolation & purification
Tumor Necrosis Factor-alpha
/ metabolism
Perilla frutescens
TNF-α
endothelial cell
inflammation
microparticle
vessel
Journal
Bioscience reports
ISSN: 1573-4935
Titre abrégé: Biosci Rep
Pays: England
ID NLM: 8102797
Informations de publication
Date de publication:
29 05 2020
29 05 2020
Historique:
received:
20
06
2019
revised:
05
05
2020
accepted:
12
05
2020
pubmed:
20
5
2020
medline:
30
3
2021
entrez:
20
5
2020
Statut:
ppublish
Résumé
Elevation of endothelial microparticles (EMPs) play an important role in the progression of inflammation-related vascular diseases such as cardiovascular diseases (CVDs). Thai perilla (Perilla frutescens) nutlets are rich in phenolic compounds and flavonoids that exert potent antioxidant and anti-inflammatory effects. We found that the ethyl acetate (EA) and ethanol (Eth) extracts of Thai perilla nutlets contain phenolic compounds such as luteolin, apigenin, chryseoriol and their glycosides, which exhibit antioxidant activity. The goal of the present study was to investigate the effects of the extracts on endothelial activation and EMPs generation in tumour necrosis factor-α (TNF-α)-induced EA.hy926 cells. We found that TNF-α (10 ng/ml) activated EA.hy926 cells and subsequently generated EMPs. Pre-treatment with the extracts significantly attenuated endothelial activation by decreasing the expression of the intracellular adhesion molecule-1 (ICAM-1) in a dose-dependent manner. Only the Eth extract showed protective effects against overproduction of interleukin-6 (IL-6) in the activated cells. Furthermore, the extracts significantly reduced TNF-α-enhanced EMPs generation in a dose-dependent manner. In conclusion, Thai perilla nutlet extracts, especially the Eth extract, may have potential to protect endothelium against vascular inflammation through the inhibition of endothelial activation and the generation of endothelial microparticles (EMPs).
Identifiants
pubmed: 32426811
pii: 224731
doi: 10.1042/BSR20192110
pmc: PMC7260356
pii:
doi:
Substances chimiques
ICAM1 protein, human
0
IL6 protein, human
0
Interleukin-6
0
Plant Extracts
0
TNF protein, human
0
Tumor Necrosis Factor-alpha
0
Intercellular Adhesion Molecule-1
126547-89-5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2020 The Author(s).
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