Using a mouse-adapted A/HK/01/68 influenza virus to analyse the impact of NS1 evolution in codons 196 and 231 on viral replication and virulence.
A549 Cells
Amino Acids
/ genetics
Animals
Cell Line
Cell Line, Tumor
Codon
/ genetics
Dogs
Evolution, Molecular
HEK293 Cells
Host-Pathogen Interactions
/ genetics
Humans
Influenza, Human
/ virology
Madin Darby Canine Kidney Cells
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Orthomyxoviridae Infections
/ virology
Viral Nonstructural Proteins
/ genetics
Virulence
/ genetics
Virus Replication
/ genetics
NS1 evolution
influenza A virus
mouse-adapted A/HK/1/68
non-structural protein 1
orthomyxoviruses
viral interferon antagonist
Journal
The Journal of general virology
ISSN: 1465-2099
Titre abrégé: J Gen Virol
Pays: England
ID NLM: 0077340
Informations de publication
Date de publication:
06 2020
06 2020
Historique:
pubmed:
18
5
2020
medline:
27
10
2020
entrez:
18
5
2020
Statut:
ppublish
Résumé
Seasonal influenza viruses circulating between 1918 and 2009 harboured two prevalent genetic variations in the NS1 coding region. A glutamic acid (E)-to-lysine (K) exchange at position 196 was reported to diminish the capacity of NS1 to control interferon induction. Furthermore, alterations at position 231 determine a carboxy-terminal extension of seven amino acids from 230 to 237 residues. Sequence analyses of NS1 of the last 90 years suggest that variations at these two positions are functionally linked. To determine the impact of the two positions on viral replication
Identifiants
pubmed: 32416749
doi: 10.1099/jgv.0.001422
doi:
Substances chimiques
Amino Acids
0
Codon
0
Viral Nonstructural Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM