Effects of Strontium-Doped β-Tricalcium Scaffold on Longitudinal Nuclear Factor-Kappa Beta and Vascular Endothelial Growth Factor Receptor-2 Promoter Activities during Healing in a Murine Critical-Size Bone Defect Model.
Animals
Bone Regeneration
Bone Substitutes
Bone and Bones
/ metabolism
Calcium Phosphates
/ chemistry
Immunohistochemistry
Mice
Mice, Transgenic
NF-kappa B
/ genetics
Phosphatidylethanolamines
/ chemistry
Promoter Regions, Genetic
Strontium
/ chemistry
Tissue Scaffolds
Vascular Endothelial Growth Factor Receptor-2
/ genetics
NF-κB
VEGFR-2
bioluminescence
large bone defects
strontium
β-tricalcium phosphate
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
01 May 2020
01 May 2020
Historique:
received:
07
04
2020
revised:
29
04
2020
accepted:
29
04
2020
entrez:
7
5
2020
pubmed:
7
5
2020
medline:
7
2
2021
Statut:
epublish
Résumé
It was hypothesized that strontium (Sr)-doped β-tricalcium phosphate (TCP)-based scaffolds have a positive effect on the regeneration of large bone defects (LBD). Readouts in our mice models were nuclear factor-kappa beta (NF-κB) activity and vascular endothelial growth factor receptor-2 (VEGFR-2) promoter activity during the healing process. A 2-mm critical-size femoral fracture was performed in transgenic NF-κB- and VEGFR-2-luciferase reporter mice. The fracture was filled with a 3D-printed β-TCP scaffold with or without Sr. A bioluminescence in-vivo imaging system was used to sequentially investigate NF-κB and VEGFR-2 expression for two months. After sacrifice, soft and osseous tissue formation in the fracture sites was histologically examined. NF-κB activity increased in the β-TCP + Sr group in the latter stage (day 40-60). VEGFR-2 activity increased in the + Sr group from days 0-15 but decreased and showed significantly less activity than the β-TCP and non-scaffold groups from days 40-60. The new bone formation and soft tissue formation in the + Sr group were significantly higher than in the β-TCP group, whereas the percentage of osseous tissue formation in the β-TCP group was significantly higher than in the β-TCP + Sr group. We analyzed longitudinal VEGFR-2 promoter activity and NF-κB activity profiles, as respective agents of angiogenesis and inflammation, during LBD healing. The extended inflammation phase and eventually more rapid resorption of scaffold caused by the addition of strontium accelerates temporary bridging of the fracture gaps. This finding has the potential to inform an improved treatment strategy for patients who suffer from osteoporosis.
Identifiants
pubmed: 32370039
pii: ijms21093208
doi: 10.3390/ijms21093208
pmc: PMC7246816
pii:
doi:
Substances chimiques
Bone Substitutes
0
Calcium Phosphates
0
NF-kappa B
0
Phosphatidylethanolamines
0
beta-tricalcium phosphate
0
dioleoyl phosphatidylethanolamine
2462-63-7
Vascular Endothelial Growth Factor Receptor-2
EC 2.7.10.1
Strontium
YZS2RPE8LE
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Références
Theranostics. 2016 Jan 01;6(3):328-41
pubmed: 26909109
J Periodontal Res. 2018 Dec;53(6):999-1008
pubmed: 30221352
J Mater Sci Mater Med. 1998 Dec;9(12):723-6
pubmed: 15348929
Calcif Tissue Int. 2019 Oct;105(4):341-352
pubmed: 31236620
Clin Orthop Relat Res. 1985 Nov;(200):100-13
pubmed: 3905103
Acta Orthop Scand. 2000 Jun;71(3):316-21
pubmed: 10919307
Acta Biomater. 2018 Apr 1;70:293-303
pubmed: 29432984
Calcif Tissue Int. 2014 Oct;95(4):349-61
pubmed: 25096517
Int J Mol Sci. 2020 Jan 20;21(2):
pubmed: 31968603
J Dent Res. 2011 Sep;90(9):1052-61
pubmed: 21248364
J Biomed Mater Res A. 2014 Oct;102(10):3677-84
pubmed: 24307071
Acta Orthop Scand. 1994 Aug;65(4):462-6
pubmed: 7976298
J Mater Sci Mater Med. 2009 Jul;20(7):1505-12
pubmed: 19267259
Mater Sci Eng C Mater Biol Appl. 2013 Apr 1;33(3):1254-60
pubmed: 23827569
Nat Med. 1998 Feb;4(2):245-7
pubmed: 9461201
J Biomater Appl. 2016 Oct;31(4):499-509
pubmed: 27164870
Blood. 2004 Jan 15;103(2):617-26
pubmed: 14512298
Tissue Eng Part B Rev. 2011 Dec;17(6):389-92
pubmed: 21902614
Wien Klin Wochenschr. 2005 Nov;117(21-22):728-38
pubmed: 16416353
J Bone Joint Surg Am. 2002 Mar;84(3):454-64
pubmed: 11886919
J Tissue Eng Regen Med. 2014 Feb;8(2):120-30
pubmed: 22495762
Bone. 2013 Jan;52(1):63-9
pubmed: 22995463
J Appl Physiol (1985). 2010 Dec;109(6):1930-8
pubmed: 20947709
Mater Sci Eng C Mater Biol Appl. 2018 Mar 1;84:159-167
pubmed: 29519425
Tissue Eng Part C Methods. 2011 May;17(5):597-606
pubmed: 21254818
Micron. 2016 Jan;80:122-34
pubmed: 26546967
Acta Biomater. 2019 Apr 1;88:527-539
pubmed: 30797105
J Vis Exp. 2017 Nov 14;(129):
pubmed: 29286432
J Bone Miner Res. 1993 May;8(5):607-15
pubmed: 8511988
Sci Rep. 2019 Nov 25;9(1):17445
pubmed: 31768003
J Dent Res. 2008 Feb;87(2):107-18
pubmed: 18218835
PLoS One. 2017 Mar 23;12(3):e0174283
pubmed: 28333972
Nat Immunol. 2005 Dec;6(12):1191-7
pubmed: 16369558
J Vis Exp. 2016 Oct 12;(116):
pubmed: 27768070
J Vis Exp. 2015 Jun 04;(100):e52770
pubmed: 26066033
Mater Sci Eng C Mater Biol Appl. 2019 May;98:1145-1158
pubmed: 30812998
Injury. 2016 Dec;47 Suppl 7:S36-S39
pubmed: 28040076
Bone. 2009 Feb;44(2):335-44
pubmed: 19013264
Materials (Basel). 2019 Jun 11;12(11):
pubmed: 31212709
Injury. 2017 Dec;48(12):2807-2813
pubmed: 29096930
Ther Adv Musculoskelet Dis. 2014 Dec;6(6):217-25
pubmed: 25435924
J Orthop Res. 1984;2(1):97-101
pubmed: 6491805
J Hand Surg Asian Pac Vol. 2018 Sep;23(3):424-429
pubmed: 30282534
Injury. 2008 Sep;39 Suppl 2:S45-57
pubmed: 18804573
Bone. 2017 Feb;95:26-32
pubmed: 27836732
J Biomed Mater Res A. 2012 Jun;100(6):1550-5
pubmed: 22419568
Arch Orthop Trauma Surg. 2008 Oct;128(10):1159-65
pubmed: 18094982
Nat Rev Rheumatol. 2015 Jan;11(1):45-54
pubmed: 25266456
Tissue Eng Part B Rev. 2008 Jun;14(2):179-86
pubmed: 18544015
Altern Ther Health Med. 2016 Mar;22(3):66-70
pubmed: 27228273
Langenbecks Arch Surg. 2014 Jun;399(5):579-88
pubmed: 24733521
Mol Med Rep. 2019 Oct;20(4):3555-3564
pubmed: 31432182
Med Sci Monit. 2019 Apr 11;25:2658-2671
pubmed: 30973161
Injury. 2005 Nov;36 Suppl 3:S20-7
pubmed: 16188545
J Am Dent Assoc. 1982 Dec;105(6):1035-8
pubmed: 6818267
J Biomed Mater Res A. 2020 Mar;108(3):633-644
pubmed: 31749231
J Orthop Res. 2010 Sep;28(9):1208-14
pubmed: 20196084
Cell Tissue Res. 2002 Sep;309(3):387-92
pubmed: 12195295
Arthritis Res Ther. 2008;10(4):212
pubmed: 18771589
BMJ. 2005 Jun 18;330(7505):1400-1
pubmed: 15961793
Mater Sci Eng C Mater Biol Appl. 2019 Jun;99:1289-1303
pubmed: 30889663
Acta Biomater. 2010 Aug;6(8):3283-91
pubmed: 20176148