Superior mechanical recovery in male and female MRL/MpJ tendons is associated with a unique genetic profile.
PCR array
regeneration
scarless healing
tendon injury
Journal
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
ISSN: 1554-527X
Titre abrégé: J Orthop Res
Pays: United States
ID NLM: 8404726
Informations de publication
Date de publication:
06 2021
06 2021
Historique:
revised:
22
04
2020
received:
15
10
2019
accepted:
24
04
2020
pubmed:
1
5
2020
medline:
3
9
2021
entrez:
1
5
2020
Statut:
ppublish
Résumé
Tendon ruptures heal by forming a mechanically inferior scar. We have shown that male Murphy Roths large (MRL/MpJ) mice exhibit improved tendon healing, suggesting that they can inform biological mechanisms that lead to effective tendon healing. As sex impacts healing, we assessed the effect of sex on tendon healing in MRL/MpJ and normal healer C57BL/6 (B6) mice and compared the associated biological environment with identify genes that may be integral to the improved healing outcome. We hypothesized that (a) male MRL/MpJ mice will heal with improved mechanical properties compared to females; and (b) that regenerative tendon healing will be associated with decreased fibrotic pathways, decreased inflammation, and increased activity of matrix metalloproteinases (MMPs). A midsubstance punch was introduced, and tendons were harvested after (a) 1 or 7 days for profiling of 84 genes; (b) 7 or 14 days for the assessment of MMP-2 and MMP-9 activity; and (c) 6 weeks for mechanical assessment. MRL/MpJ tendons healed with the better restoration of mechanical properties than B6 tendons. Sex did not affect the mechanical properties of healing B6 or MRL/MpJ tendons. Comparison of the gene expression profiles in the context of the mechanical outcome revealed several differences between MRL/MpJ and B6 tendon healing, including, lower inflammation, an earlier higher expression of TGF-β-related genes that diminish by 7 days, and genes associated with enhanced cell migration in MRL/MpJ in comparison to B6 tendons. We expect that the timecourse and expression levels of these genes in scarless MRL/MpJ tendon healing represent the balanced environment that leads to improved tendon healing.
Identifiants
pubmed: 32352601
doi: 10.1002/jor.24705
pmc: PMC7606617
mid: NIHMS1611978
doi:
Substances chimiques
Matrix Metalloproteinase 2
EC 3.4.24.24
Matrix Metalloproteinase 9
EC 3.4.24.35
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1344-1354Subventions
Organisme : NIAMS NIH HHS
ID : R01 AR052743
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR068301
Pays : United States
Organisme : NIAMS NIH HHS
ID : AR068301
Pays : United States
Organisme : NIAMS NIH HHS
ID : AR52743
Pays : United States
Organisme : NIAMS NIH HHS
ID : AR068301
Pays : United States
Organisme : NIAMS NIH HHS
ID : AR52743
Pays : United States
Informations de copyright
© 2020 Orthopaedic Research Society. Published by Wiley Periodicals LLC.
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