Impacts of different synthetic polymers on vitrification of ovarian tissue.
Cryoprotective agents
In vitro culture
Ovarian tissue
Synthetic polymers
Vitrification
Journal
Cryobiology
ISSN: 1090-2392
Titre abrégé: Cryobiology
Pays: Netherlands
ID NLM: 0006252
Informations de publication
Date de publication:
06 2020
06 2020
Historique:
received:
26
02
2020
revised:
18
04
2020
accepted:
18
04
2020
pubmed:
28
4
2020
medline:
31
12
2020
entrez:
28
4
2020
Statut:
ppublish
Résumé
Type and concentration of cryoprotective agents (CPAs) are important factors which influence the likelihood of a successful ovarian tissue vitrification outcome. In an attempt to address this factor, the present study was conducted to evaluate the impacts of different synthetic polymers (Supercool X-1000, Supercool Z-1000 and PVP K-12) on vitrification of bovine ovarian tissue. From each ovarian pair, fragments were recovered and immediately fixed for analysis (fresh control) or submitted to vitrification, either or not followed by in vitro culture for one or five days. Vitrification was performed using the ovarian tissue cryosystem (OTC) system. The ovarian tissues were intended for histological and viability analysis [Reactive oxygen species (ROS) production and degenerate cells assay (Ethidium homodimer-1)], as well as immunolocalization of AQP3 and AQP9 were measured. The results showed that during almost all the periods after warming, in treatment groups which contain polymer (X-1000, Z-1000 and PVP), the percentage of morphologically normal follicles was the highest in the X-1000 samples. Furthermore, post-thawed X-1000 group revealed stronger labeling for AQP9 in primordial and transitional follicles, when compared with others. However, morphology after cryopreservation did not correlate with follicle viability and function where the levels of degeneration and tissue damage of PVP K-12 group were lower in comparison with X-1000 group and only in PVP K-12 group, ROS level was similar to that of the fresh control group. We believe that in addition to permeating CPAs, the addition of one (Supercool X-1000) or maybe a combination (Supercool X-1000 and PVP K-12) of non-permeating polymers could be useful to improve the outcome for vitrified bovine ovarian tissue.
Identifiants
pubmed: 32339491
pii: S0011-2240(20)30078-X
doi: 10.1016/j.cryobiol.2020.04.007
pmc: PMC7660932
mid: NIHMS1625468
pii:
doi:
Substances chimiques
Cryoprotective Agents
0
Polymers
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
66-72Subventions
Organisme : NIH HHS
ID : P51 OD011092
Pays : United States
Organisme : NICHD NIH HHS
ID : R01 HD083930
Pays : United States
Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest There is no competing interest.
Références
Theriogenology. 2006 Apr 1;65(6):1200-14
pubmed: 16169072
Cryobiology. 2018 Apr;81:17-26
pubmed: 29518373
Cryobiology. 2017 Dec;79:29-36
pubmed: 28987775
Biomacromolecules. 2009 Feb 9;10(2):328-33
pubmed: 19072300
Anim Reprod Sci. 2009 Feb;110(3-4):207-21
pubmed: 18295986
Acta Histochem. 2014 Jun;116(5):831-7
pubmed: 24629225
Cryobiology. 2011 Dec;63(3):256-62
pubmed: 21985766
Cryobiology. 2004 Feb;48(1):22-35
pubmed: 14969679
Anim Reprod Sci. 2013 May;138(3-4):220-7
pubmed: 23522695
Int J Pharm. 2013 Dec 5;457(2):423-7
pubmed: 23305867
J Turk Ger Gynecol Assoc. 2012 Sep 01;13(3):196-203
pubmed: 24592038
Eur J Obstet Gynecol Reprod Biol. 2008 Aug;139(2):193-8
pubmed: 18455864
Reproduction. 2010 Jun;139(6):971-8
pubmed: 20207724
Hum Reprod. 2012 Nov;27(11):3249-58
pubmed: 22888166
Reprod Biomed Online. 2010 Oct;21(4):501-9
pubmed: 20817609
Eur J Obstet Gynecol Reprod Biol. 2010 Jul;151(1):70-1
pubmed: 20423751
Cell Tissue Bank. 2014 Dec;15(4):591-601
pubmed: 24610241
Lancet. 2014 Oct 4;384(9950):1302-10
pubmed: 25283571
Fertil Steril. 2012 Nov;98(5):1291-8.e1-2
pubmed: 22883570
Theriogenology. 2016 May;85(8):1457-67
pubmed: 26876055
Cell Tissue Res. 2016 Aug;365(2):415-24
pubmed: 26975215
Cryo Letters. 2002 Sep-Oct;23(5):333-44
pubmed: 12447493
Cryobiology. 2002 Feb;44(1):14-23
pubmed: 12061844
Nat Commun. 2014;5:3244
pubmed: 24488146
Cryobiology. 2007 Feb;54(1):1-12
pubmed: 17166492
Reprod Biomed Online. 2011 Aug;23(2):160-86
pubmed: 21676653
Cryobiology. 2012 Aug;65(1):1-11
pubmed: 22569078
J Histochem Cytochem. 2009 Jan;57(1):61-7
pubmed: 18824632
Hum Reprod. 2010 May;25(5):1199-205
pubmed: 20185513
Womens Health (Lond). 2016 Jun;12(3):275-8
pubmed: 27188229
Zygote. 2010 May;18(2):145-53
pubmed: 19857362
Fertil Steril. 2010 Nov;94(6):2088-95
pubmed: 20171613
Cryobiology. 2000 May;40(3):228-36
pubmed: 10860622
Fertil Steril. 2006 Apr;85 Suppl 1:1150-6
pubmed: 16616087
Cryo Letters. 2014 Jul-Aug;35(4):286-92
pubmed: 25282495