Infertility-Causing Haploinsufficiency Reveals TRIM28/KAP1 Requirement in Spermatogonia.


Journal

Stem cell reports
ISSN: 2213-6711
Titre abrégé: Stem Cell Reports
Pays: United States
ID NLM: 101611300

Informations de publication

Date de publication:
12 05 2020
Historique:
received: 08 01 2020
revised: 13 03 2020
accepted: 17 03 2020
pubmed: 18 4 2020
medline: 17 4 2021
entrez: 18 4 2020
Statut: ppublish

Résumé

Spermatogenesis relies on exquisite stem cell homeostasis, the carefully balanced self-renewal and differentiation of spermatogonial stem cells (SSCs). Disturbing this equilibrium will likely manifest through sub- or infertility, a global health issue with often idiopathic presentation. In this respect, disease phenotypes caused by haploinsufficiency of otherwise vital developmental genes are of particular interest. Here, we show that mice heterozygous for Trim28, an essential epigenetic regulator, suffer gradual testicular degeneration. Contrary to previous reports we detect Trim28 expression in spermatogonia, albeit at low levels. Further reduction through Trim28 heterozygosity increases the propensity of SSCs to differentiate at the cost of self-renewal.

Identifiants

pubmed: 32302554
pii: S2213-6711(20)30103-X
doi: 10.1016/j.stemcr.2020.03.013
pmc: PMC7220855
pii:
doi:

Substances chimiques

Trim28 protein, mouse EC 2.3.2.27
Tripartite Motif-Containing Protein 28 EC 2.3.2.27

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

818-827

Informations de copyright

Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.

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Auteurs

Joel H L Tan (JHL)

Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A(∗)STAR), Singapore 138673, Singapore; NUS Graduate School for Integrative Sciences and Engineering (NGS), National University of Singapore, Singapore 119077, Singapore.

Heike Wollmann (H)

Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A(∗)STAR), Singapore 138673, Singapore.

Ans M M van Pelt (AMM)

Center for Reproductive Medicine, Amsterdam Research Institute Reproduction and Development, Academic Medical Centre, University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, the Netherlands.

Philipp Kaldis (P)

Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A(∗)STAR), Singapore 138673, Singapore.

Daniel M Messerschmidt (DM)

Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A(∗)STAR), Singapore 138673, Singapore. Electronic address: danielm@imcb.a-star.edu.sg.

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Classifications MeSH