Regulation of aberrantly expressed SERPINH1 by antitumor miR-148a-5p inhibits cancer cell aggressiveness in gastric cancer.
Cadherins
/ genetics
Cell Line, Tumor
Cell Proliferation
/ genetics
Databases, Genetic
Down-Regulation
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
/ genetics
Gene Knockdown Techniques
HSP47 Heat-Shock Proteins
/ genetics
Humans
MicroRNAs
/ genetics
Potassium Channels, Voltage-Gated
/ genetics
Procollagen-Proline Dioxygenase
/ genetics
Prognosis
RNA, Small Interfering
RNA-Induced Silencing Complex
/ genetics
Stomach Neoplasms
/ genetics
Survival Rate
Thrombospondins
/ genetics
Transaminases
/ genetics
Journal
Journal of human genetics
ISSN: 1435-232X
Titre abrégé: J Hum Genet
Pays: England
ID NLM: 9808008
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
21
02
2020
accepted:
12
03
2020
revised:
12
03
2020
pubmed:
3
4
2020
medline:
18
11
2020
entrez:
3
4
2020
Statut:
ppublish
Résumé
RNA-sequencing-based microRNA (miRNA) expression signatures have revealed that miR-148a-5p (the passenger strand of the miR-148a-duplex) is downregulated in various kinds of cancer tissues. Analysis of The Cancer Genome Atlas (TCGA) database showed that low expression of miR-148a-5p was predictive of a lower survival rate (p = 0.041) in patients with gastric cancer (GC). Downregulation of miR-148a-5p was confirmed in GC clinical specimens, and its ectopic expression attenuated GC cell proliferation. Our search for miRNA target genes identified a total of 18 oncogenic targets of miR-148a-5p in GC cells. Among these targets, high expression levels of six genes (THBS2, P4HA3, SERPINH1, CDH11, BCAT1, and KCNG3) were closely associated with a poor prognosis (10-year survival rates) in GC patients (p < 0.05) according to TCGA database analyses. Furthermore, we focused on SERPINH1 as a chaperone protein involved in collagen folding in humans. Aberrant expression of SERPINH1 (mRNA and protein levels) was confirmed in GC clinical specimens. Knockdown assays of SERPINH1 using siRNAs resulted in inhibition of the aggressive phenotype of GC cells. Exploring the molecular networks controlled by miRNAs (including miRNA passenger strands) will broaden our understanding of the molecular pathogenesis of GC.
Identifiants
pubmed: 32235846
doi: 10.1038/s10038-020-0746-6
pii: 10.1038/s10038-020-0746-6
doi:
Substances chimiques
Cadherins
0
HSP47 Heat-Shock Proteins
0
KCNG3 protein, human
0
MIRN148 microRNA, human
0
MicroRNAs
0
Potassium Channels, Voltage-Gated
0
RNA, Small Interfering
0
RNA-Induced Silencing Complex
0
SERPINH1 protein, human
0
Thrombospondins
0
thrombospondin 2
0
osteoblast cadherin
156621-71-5
P4HA3 protein, human
EC 1.14.11.2
Procollagen-Proline Dioxygenase
EC 1.14.11.2
BCAT1 protein, human
EC 2.6.1.
Transaminases
EC 2.6.1.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
647-656Références
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