The Synaptic Vesicle Priming Protein CAPS-1 Shapes the Adaptation of Sensory Evoked Responses in Mouse Visual Cortex.

Acute Brain slice Patch technique Ca(2+)- dependent Activator Protein for Secretion (CAPS) Optogenetic Stimulation Sensory Adaptation Synaptic Vesicle Priming Visual Cortex in-vivo patch clamp technique

Journal

Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691

Informations de publication

Date de publication:
10 03 2020
Historique:
received: 07 12 2018
revised: 22 10 2019
accepted: 10 02 2020
entrez: 12 3 2020
pubmed: 12 3 2020
medline: 24 3 2021
Statut: ppublish

Résumé

Short-term plasticity gates information transfer across neuronal synapses and is thought to be involved in fundamental brain processes, such as cortical gain control and sensory adaptation. Neurons employ synaptic vesicle priming proteins of the CAPS and Munc13 families to shape short-term plasticity in vitro, but the relevance of this phenomenon for information processing in the intact brain is unknown. By combining sensory stimulation with in vivo patch-clamp recordings in anesthetized mice, we show that genetic deletion of CAPS-1 in thalamic neurons results in more rapid adaptation of sensory-evoked subthreshold responses in layer 4 neurons of the primary visual cortex. Optogenetic experiments in acute brain slices further reveal that the enhanced adaptation is caused by more pronounced short-term synaptic depression. Our data indicate that neurons engage CAPS-family priming proteins to shape short-term plasticity for optimal sensory information transfer between thalamic and cortical neurons in the intact brain in vivo.

Identifiants

pubmed: 32160535
pii: S2211-1247(20)30206-0
doi: 10.1016/j.celrep.2020.02.045
pii:
doi:

Substances chimiques

Cadps protein, mouse 0
Calcium-Binding Proteins 0
Nerve Tissue Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

3261-3269.e4

Informations de copyright

Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Interests All authors declare no competing interests.

Auteurs

Dennis B Nestvogel (DB)

Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany; International Max Planck Research School for Neuroscience at the University of Göttingen, 37075 Göttingen, Germany. Electronic address: dennisnestvogel@gmail.com.

Ricardo Martins Merino (RM)

International Max Planck Research School for Neuroscience at the University of Göttingen, 37075 Göttingen, Germany; Theoretical Neurophysics Group, Max Planck Institute for Dynamics and Self Organization, 37077 Göttingen, Germany; Department of Molecular Biology of Neuronal Signals, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany.

Carolina Leon-Pinzon (C)

Theoretical Neurophysics Group, Max Planck Institute for Dynamics and Self Organization, 37077 Göttingen, Germany; Department of Molecular Biology of Neuronal Signals, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany; Campus Institute for Dynamics of Biological Networks, 37075 Göttingen, Germany.

Manuel Schottdorf (M)

Princeton Neuroscience Institute, Princeton University, Princeton, NJ 08544, USA.

ChoongKu Lee (C)

Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany.

Cordelia Imig (C)

Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany.

Nils Brose (N)

Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany.

Jeong-Seop Rhee (JS)

Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany. Electronic address: rhee@em.mpg.de.

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Classifications MeSH