Bat guano-dwelling microbes and antimicrobial properties of the pygidial gland secretion of a troglophilic ground beetle against them.
Bat guano
Karstic cave
Laemostenus (Pristonychus) punctatus
Microbial diversity
Pygidial glands
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
May 2020
May 2020
Historique:
received:
07
11
2019
accepted:
23
02
2020
revised:
26
01
2020
pubmed:
7
3
2020
medline:
9
1
2021
entrez:
7
3
2020
Statut:
ppublish
Résumé
Bat guano is an important source of microbial diversity in caves and can be a source of potential pathogens. Laemostenus (Pristonychus) punctatus is a guanophilic ground beetle species, which pygidial gland secretion exhibits action against pathogenic and other microbes. The distribution and diversity of microbes in bat guano from a karstic cave were determined in this study. Additionally, antimicrobial activity of the pygidial gland secretion of L. (P.) punctatus against guano-dwelling microbes was tested; minimal inhibitory concentration (MIC) and chemical composition of the secretion were analyzed. In total, 63 different bacterial species and 16 fungal morphotypes were isolated from guano samples by the cultivation method and confirmed using and phenotypic characterization and molecular identification. There was a difference in the composition of certain microorganisms between the sampling points (cave locations) and between the guano layers. The largest number of bacterial isolates belongs to the genera Lysinibacillus and Paenibacillus, while Pseudomonas species were highly abundant at the innermost sampling point. For the guanophilic fungi, the majority are ascomycetes, with Penicillium and Aspergillus as the most dominant genera. Meyerozyma guilliermondii was the only yeast species found in the guano samples. The most sensitive isolates were Enterococcus eurekensis (MIC 0.007 mg/mL) and Escherichia fergusonii (MIC 0.028 mg/mL). The most sensitive fungal isolates were M. guilliermondii, Penicillium expansum, and Trichoderma harzianum (MIC 0.15 mg/mL). This study opens a new possibility for better understanding of ecological relations between microorganisms and troglophilic ground beetles and for detailed investigations of morpho-anatomical aspects of pygidial glands.
Identifiants
pubmed: 32140841
doi: 10.1007/s00253-020-10498-y
pii: 10.1007/s00253-020-10498-y
doi:
Substances chimiques
Anti-Bacterial Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
4109-4126Subventions
Organisme : Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja
ID : ON173026
Organisme : Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja
ID : ON173032
Organisme : Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja
ID : ON173038
Organisme : Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja
ID : III43001
Organisme : Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja
ID : ON172053
Organisme : Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja
ID : III45016
Organisme : Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja
ID : ON171038
Organisme : Serbian Ministry of Education, Science and Technological Development and the Ministry of Science and Technology of the People's Republic of China
ID : ''Mimetics of Insects for Sensing and Security'', I-2
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