Indoxyl Acetate as a Substrate for Analysis of Lipase Activity.
Journal
International journal of analytical chemistry
ISSN: 1687-8760
Titre abrégé: Int J Anal Chem
Pays: Egypt
ID NLM: 101519424
Informations de publication
Date de publication:
2019
2019
Historique:
received:
22
07
2019
revised:
10
10
2019
accepted:
11
11
2019
entrez:
31
12
2019
pubmed:
31
12
2019
medline:
31
12
2019
Statut:
epublish
Résumé
Lipases play a crucial role in metabolism of microbes, fungi, plants, and animals, and in analytical chemistry, they are often used in detection of fats and triglycerides. Determination of lipase activity is also important in toxicology, when lipase activity can be both increased and decreased by organophosphates and other pesticides and in medicine for diagnosis of heart diseases. The standard method for lipase activity determination is based on cleaving ester bonds in lipase buffer containing Tween. Our aim was to find a method with faster and more sensitive response. It is known that acetylcholinesterase belongs to the same group of hydrolases enzymes as lipases and it cleaves indoxyl acetate, so we assume indoxyl acetate could report a similar reaction with lipase. Our method is based on indoxyl acetate as a substrate for lipase, where indoxyl acetate is cleaved by lipase to indoxyl and acetate moiety and blue indigo is created. The method was optimized for different times and amount of enzyme and compared with the standard Tween assay. The calibration curve measured in reaction time 20 minutes with 10
Identifiants
pubmed: 31885593
doi: 10.1155/2019/8538340
pmc: PMC6914949
doi:
Types de publication
Journal Article
Langues
eng
Pagination
8538340Informations de copyright
Copyright © 2019 Tomas Valek et al.
Déclaration de conflit d'intérêts
The authors declare that they have no conflicts of interest regarding the publication of this article.
Références
Protein Expr Purif. 2006 Jun;47(2):516-23
pubmed: 16380267
Protein Expr Purif. 2013 Mar;88(1):67-79
pubmed: 23202292
J Mol Med (Berl). 2002 Dec;80(12):753-69
pubmed: 12483461
Trends Biotechnol. 2002 Oct;20(10):433-7
pubmed: 12220906
J Comput Chem. 2004 Oct;25(13):1605-12
pubmed: 15264254
Enzyme Microb Technol. 2018 Jan;108:42-52
pubmed: 29108626
Chembiochem. 2015 Mar 2;16(4):677-82
pubmed: 25648400
Z Lebensm Unters Forsch. 1985 Oct;181(4):271-5
pubmed: 4072422
J Genet Eng Biotechnol. 2017 Dec;15(2):369-377
pubmed: 30647675
Org Biomol Chem. 2016 Sep 26;14(38):9146-9150
pubmed: 27714153
Anal Biochem. 2017 Aug 1;530:104-106
pubmed: 28502711
Adv Colloid Interface Sci. 2009 Mar-Jun;147-148:237-50
pubmed: 18691682
Biosensors (Basel). 2014 Sep 09;4(3):301-17
pubmed: 25587424
Recent Pat Biotechnol. 2019;13(1):45-56
pubmed: 30370868
Mol Biotechnol. 2010 Oct;46(2):168-75
pubmed: 20387014
Chem Biol Interact. 2010 Sep 6;187(1-3):10-22
pubmed: 20138030
Interdiscip Toxicol. 2014 Dec;7(4):215-8
pubmed: 26109903
Lipids. 1978 Jun;13(6):433-7
pubmed: 672483
Acta Biochim Pol. 1993;40(4):563-7
pubmed: 8140833
J Bacteriol. 1996 Apr;178(7):2060-4
pubmed: 8606184
Mini Rev Med Chem. 2015;15(14):1209-16
pubmed: 26156413
BMC Genomics. 2010 Feb 19;11:123
pubmed: 20170513
Am J Emerg Med. 2009 Nov;27(9):1117-24
pubmed: 19931761
Biochemistry. 2008 Sep 9;47(36):9553-64
pubmed: 18702514
Appl Biochem Biotechnol. 2012 Jan;166(2):486-520
pubmed: 22072143
Int J Mol Sci. 2011;12(4):2631-40
pubmed: 21731462
Acta Pharmacol Toxicol (Copenh). 1983 Aug;53(2):121-4
pubmed: 6624479
Colloids Surf B Biointerfaces. 2009 May 1;70(2):238-42
pubmed: 19195852
Biomed Res Int. 2013;2013:329121
pubmed: 24106701
Arh Hig Rada Toksikol. 2017 Dec 20;68(4):261-275
pubmed: 29337682
Biochem J. 1999 Oct 1;343 Pt 1:177-83
pubmed: 10493927
Biochim Biophys Acta. 2005 Oct 30;1726(1):67-74
pubmed: 16154275
Enzyme Microb Technol. 2014 Jun 10;60:1-8
pubmed: 24835093