Bio-assay of Acintobacter baumannii using DNA conjugated with gold nano-star: A new platform for microorganism analysis.
Acinetobacter baumannii
Bacteria monitoring
Bio-assay
Gold nanoparticle
Microorganism
Journal
Enzyme and microbial technology
ISSN: 1879-0909
Titre abrégé: Enzyme Microb Technol
Pays: United States
ID NLM: 8003761
Informations de publication
Date de publication:
Feb 2020
Feb 2020
Historique:
received:
24
08
2019
revised:
07
11
2019
accepted:
08
11
2019
entrez:
26
12
2019
pubmed:
26
12
2019
medline:
1
5
2020
Statut:
ppublish
Résumé
Acinetobacter baumannii is a non-motile, gram-negative member of the gamma proteobacteria. A specific and sensitive approach was established for the detection of Acintobacter baumannii via DNA based bio-assay. In this study, gold nano-star was synthesized and used for bio-conjugation with pDNA toward the detection of target sequences. Synthesized probe (5' TTG TGA ACT ATT TAC GTC AGC ATG C3') of Acinetobacter baumannii was found with excellent sensitivity. After the hybridization of pDNA with cDNA, target DNA (5' GCA TGC TGA CGT AAA TAG TTC ACA A 3') was easily measured. According to ultra-sensitivity of the engineered optical DNA-based bio-assay, it is potentially applied in the bacterial detection of the environmental and clinical specimens. Here, the selection of engineered biosensor in the presence of two mismatch sequences was investigated. The results indicated an acceptable choice for DNA-based assays. The low limit of quantification (LLOQ) of genosensor was obtained as 1 fM. The present study is a very important diagnostic examination to recognize Acinetobacter baumannii, which can be a best alternative to the traditional methods.
Identifiants
pubmed: 31874682
pii: S0141-0229(19)30204-2
doi: 10.1016/j.enzmictec.2019.109466
pii:
doi:
Substances chimiques
DNA, Bacterial
0
Gold
7440-57-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
109466Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.