Quantitative determination of H


Journal

Analytical biochemistry
ISSN: 1096-0309
Titre abrégé: Anal Biochem
Pays: United States
ID NLM: 0370535

Informations de publication

Date de publication:
15 02 2020
Historique:
received: 18 09 2019
revised: 21 11 2019
accepted: 07 12 2019
pubmed: 13 12 2019
medline: 29 12 2020
entrez: 13 12 2019
Statut: ppublish

Résumé

The abnormal concentrations or absence of biomolecules (e.g., proteins) in blood can further be used in diagnosis of a particular pathology at an early stage. Current studies are intensely focusing on the analysis of interaction and detection of biomolecules via point-of-care systems (POCs), allowing miniaturized and parallelized reactions, simultaneously. Recent developments have shown that the collaboration of electrochemical sensing techniques and POCs to overcome challenging problems in health-care settings provides new approaches in diagnosis and treatment of diseases. The aim of this study was to adapt the alanine aminotransferase (ALT) enzyme to the platinum (Pt) thin film electrode system and quantitatively determine the enzyme levels via enzymatically generated H

Identifiants

pubmed: 31830435
pii: S0003-2697(19)30906-6
doi: 10.1016/j.ab.2019.113538
pii:
doi:

Substances chimiques

Platinum 49DFR088MY
Hydrogen Peroxide BBX060AN9V
Alanine Transaminase EC 2.6.1.2

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

113538

Informations de copyright

Copyright © 2019 Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest Authors have declared no conflict of interest.

Auteurs

Ecem Saygili (E)

Department of Bioengineering, Faculty of Engineering, Ege University, 35100, Bornova, Izmir, Turkey.

Beyza Orakci (B)

Department of Bioengineering, Faculty of Engineering, Ege University, 35100, Bornova, Izmir, Turkey.

Melisa Koprulu (M)

Department of Bioengineering, Faculty of Engineering, Ege University, 35100, Bornova, Izmir, Turkey.

Alper Demirhan (A)

Solar Biyoteknoloji Ltd. (SolarBiotec), 35530, Bayrakli, Izmir, Turkey.

Esra Ilhan-Ayisigi (E)

Department of Bioengineering, Faculty of Engineering, Ege University, 35100, Bornova, Izmir, Turkey; Genetic and Bioengineering Department, Faculty of Engineering and Architecture, Ahi Evran University, Kirsehir, Turkey.

Yalin Kilic (Y)

Department of Biomedical Engineering, Faculty of Engineering, Izmir University of Economics, 35330, Balcova, Izmir, Turkey.

Ozlem Yesil-Celiktas (O)

Department of Bioengineering, Faculty of Engineering, Ege University, 35100, Bornova, Izmir, Turkey. Electronic address: ozlem.yesil.celiktas@ege.edu.tr.

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Classifications MeSH