Quantitative determination of H
Alanine aminotransferase
Differential pulse voltammetry
Electrochemical sensing
Hydrogen peroxide
Potentiostat
Thin-film electrodes
Journal
Analytical biochemistry
ISSN: 1096-0309
Titre abrégé: Anal Biochem
Pays: United States
ID NLM: 0370535
Informations de publication
Date de publication:
15 02 2020
15 02 2020
Historique:
received:
18
09
2019
revised:
21
11
2019
accepted:
07
12
2019
pubmed:
13
12
2019
medline:
29
12
2020
entrez:
13
12
2019
Statut:
ppublish
Résumé
The abnormal concentrations or absence of biomolecules (e.g., proteins) in blood can further be used in diagnosis of a particular pathology at an early stage. Current studies are intensely focusing on the analysis of interaction and detection of biomolecules via point-of-care systems (POCs), allowing miniaturized and parallelized reactions, simultaneously. Recent developments have shown that the collaboration of electrochemical sensing techniques and POCs to overcome challenging problems in health-care settings provides new approaches in diagnosis and treatment of diseases. The aim of this study was to adapt the alanine aminotransferase (ALT) enzyme to the platinum (Pt) thin film electrode system and quantitatively determine the enzyme levels via enzymatically generated H
Identifiants
pubmed: 31830435
pii: S0003-2697(19)30906-6
doi: 10.1016/j.ab.2019.113538
pii:
doi:
Substances chimiques
Platinum
49DFR088MY
Hydrogen Peroxide
BBX060AN9V
Alanine Transaminase
EC 2.6.1.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
113538Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest Authors have declared no conflict of interest.