Isl1 Regulation of Nkx2.1 in the Early Foregut Epithelium Is Required for Trachea-Esophageal Separation and Lung Lobation.
Animals
Body Patterning
/ genetics
Digestive System
/ metabolism
Endoderm
/ metabolism
Epithelium
/ metabolism
Esophagus
/ metabolism
Gene Expression Regulation, Developmental
/ genetics
LIM-Homeodomain Proteins
/ genetics
Lung
/ metabolism
Mice
Morphogenesis
/ physiology
Thyroid Nuclear Factor 1
/ genetics
Trachea
/ metabolism
Transcription Factors
/ genetics
Xenopus
EA/TEF
Isl1
Nkx2.1
Sox2
esophageal atresia
foregut separation
lobation
lung development
Journal
Developmental cell
ISSN: 1878-1551
Titre abrégé: Dev Cell
Pays: United States
ID NLM: 101120028
Informations de publication
Date de publication:
16 12 2019
16 12 2019
Historique:
received:
04
05
2019
revised:
18
09
2019
accepted:
04
11
2019
pubmed:
10
12
2019
medline:
11
6
2020
entrez:
10
12
2019
Statut:
ppublish
Résumé
The esophagus and trachea arise from the dorsal and ventral aspects of the anterior foregut, respectively. Abnormal trachea-esophageal separation leads to the common birth defect esophageal atresia with or without trachea-esophageal fistula (EA/TEF). Yet the underlying cellular mechanisms remain unknown. Here, we combine Xenopus and mouse genetic models to identify that the transcription factor Isl1 orchestrates trachea-esophageal separation through modulating a specific epithelial progenitor cell population (midline epithelial cells [MECs], Isl1+ Nkx2.1+ Sox2+) located at the dorsal-ventral boundary of the foregut. Lineage tracing experiments show that MECs contribute to both tracheal and esophageal epithelium, and Isl1 is required for Nkx2.1 transcription in MECs. Deletion of the chromosomal region spanning the ISL1 gene has been found in patients with abnormal trachea-esophageal separation. Our studies thus provide definitive evidence that ISL1 is a critical player in the process of foregut morphogenesis, acting in a small progenitor population of boundary cells.
Identifiants
pubmed: 31813798
pii: S1534-5807(19)30910-4
doi: 10.1016/j.devcel.2019.11.002
pmc: PMC6919560
mid: NIHMS1543851
pii:
doi:
Substances chimiques
LIM-Homeodomain Proteins
0
Nkx2-1 protein, mouse
0
Thyroid Nuclear Factor 1
0
Transcription Factors
0
insulin gene enhancer binding protein Isl-1
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
675-683.e4Subventions
Organisme : NIDDK NIH HHS
ID : R01 DK100342
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL132996
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK120650
Pays : United States
Organisme : NIAID NIH HHS
ID : R24 AI059830
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK113144
Pays : United States
Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.
Références
Development. 2019 Feb 11;146(3):
pubmed: 30696710
Dev Dyn. 2007 Mar;236(3):746-54
pubmed: 17260385
Front Pediatr. 2014 May 05;2:39
pubmed: 24829898
Cell Stem Cell. 2018 Oct 4;23(4):516-529.e5
pubmed: 30244870
Nature. 1997 Jan 16;385(6613):257-60
pubmed: 9000074
Differentiation. 2006 Sep;74(7):422-37
pubmed: 16916379
Cell Stem Cell. 2011 Oct 4;9(4):317-29
pubmed: 21982232
Mol Cell Biol. 2007 Mar;27(6):2155-65
pubmed: 17220277
Development. 2011 Mar;138(5):971-81
pubmed: 21303850
Nature. 2009 Jul 2;460(7251):113-7
pubmed: 19571884
Dev Cell. 2003 Dec;5(6):877-89
pubmed: 14667410
Cell Rep. 2016 Jun 28;16(1):66-78
pubmed: 27320915
Nature. 2017 Oct 26;550(7677):529-533
pubmed: 29019984
Development. 2008 Jun;135(11):1981-90
pubmed: 18434421
Proc Natl Acad Sci U S A. 2009 Sep 22;106(38):16287-92
pubmed: 19805295
J Pediatr. 2016 Sep;176:99-104.e1
pubmed: 27318379
Semin Cell Dev Biol. 2017 Jun;66:25-35
pubmed: 28007661
Eur J Hum Genet. 2010 Nov;18(11):1265-8
pubmed: 20551993
Arch Dis Child. 2012 Sep;97(9):808-11
pubmed: 22753768
J Biol Chem. 2015 Nov 6;290(45):27297-310
pubmed: 26378236
Dev Biol. 1999 May 1;209(1):60-71
pubmed: 10208743
Dev Dyn. 2015 Jan;244(1):69-85
pubmed: 25156440
Neuron. 2011 Sep 22;71(6):995-1013
pubmed: 21943598
Nat Genet. 1998 Sep;20(1):54-7
pubmed: 9731531
Development. 2008 May;135(9):1625-34
pubmed: 18367557
Nat Genet. 1998 Sep;20(1):58-61
pubmed: 9731532
Radiol Res Pract. 2011;2011:460890
pubmed: 22091379
Cell. 2006 Dec 15;127(6):1151-65
pubmed: 17123592
Dev Cell. 2018 Mar 26;44(6):679-693.e5
pubmed: 29587142
Dev Cell. 2010 Jan 19;18(1):8-23
pubmed: 20152174
Proc Natl Acad Sci U S A. 2013 Nov 26;110(48):19444-9
pubmed: 24218621
Cell. 2004 Aug 20;118(4):517-28
pubmed: 15315763
Dev Cell. 2009 Aug;17(2):290-8
pubmed: 19686689
Development. 2003 Jul;130(13):3063-74
pubmed: 12756187
PLoS Genet. 2014 Apr 24;10(4):e1004280
pubmed: 24763339
Dev Biol. 2014 Jul 1;391(1):111-24
pubmed: 24631216
Mol Cell Biol. 1994 Sep;14(9):5671-81
pubmed: 8065304
Dev Dyn. 1996 Nov;207(3):235-52
pubmed: 8922523
Development. 2007 Jul;134(13):2521-31
pubmed: 17522155
Development. 2002 Oct;129(20):4831-42
pubmed: 12361974
Nat Genet. 1999 Jan;21(1):70-1
pubmed: 9916792
Development. 2009 Jun;136(11):1899-907
pubmed: 19403656
J Pediatr Surg. 2002 Aug;37(8):1205-7
pubmed: 12149704
J Pediatr. 2008 Sep;153(3):396-401
pubmed: 18534205
Eur Respir J. 2010 Nov;36(5):1106-12
pubmed: 20351029
Wiley Interdiscip Rev Dev Biol. 2015 Jul-Aug;4(4):419-30
pubmed: 25727889