Dose-response of different dietary leucine levels on growth performance and amino acid metabolism in piglets differing for aminoadipate-semialdehyde synthase genotypes.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
06 12 2019
Historique:
received: 22 07 2019
accepted: 08 11 2019
entrez: 8 12 2019
pubmed: 8 12 2019
medline: 12 11 2020
Statut: epublish

Résumé

Dose-response studies of dietary leucine (Leu) in weaners are needed for a proper diet formulation. Dietary Leu effect was assessed in a 3-weeks dose-response trial with a 2 (genotype) x 5 (diets) factorial arrangement on one-hundred weaned pigs (9 to 20 kg body weight (BW)). Pigs differed for a polymorphism at the aminoadipate-semialdehyde synthase (AASS) gene, involved in lysine (Lys) metabolism. Pigs received experimental diets (d7 to d28) differing for the standardized ileal digestible (SID) Leu:Lys: 70%, 85%, 100%, 115%, 130%. Daily feed intake (ADFI), daily gain (ADG) and feed:gain (F:G) in all pigs and ADG and F:G in two classes of BW were analyzed using regression analysis with curvilinear-plateau (CLP) and linear quadratic function (LQ) models. Amino acid (AA) concentrations in plasma, liver, muscle and urine were determined. AASS genotype did not affect the parameters. Dietary Leu affected performance parameters, with a maximum response for ADG and F:G between 100.5% and 110.7% SID Leu:Lys, higher than the usually recommended one, and between 110.5% and 115.4% and between 94.9% and 110.2% SID Leu:Lys for ADG for light and heavy pigs respectively. AA variations in tissues highlighted Leu role in protein synthesis and its influence on the other branched chain AAs.

Identifiants

pubmed: 31811253
doi: 10.1038/s41598-019-55006-z
pii: 10.1038/s41598-019-55006-z
pmc: PMC6898585
doi:

Substances chimiques

Amino Acids 0
L-Aminoadipate-Semialdehyde Dehydrogenase EC 1.2.1.31
Leucine GMW67QNF9C

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

18496

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Auteurs

Micol Bertocchi (M)

Department of Agricultural, Environmental and Food Sciences, University of Molise, Campobasso, Italy.

Paolo Bosi (P)

Department of Agricultural and Food Sciences, University of Bologna, 40127, Bologna, Italy.

Diana Luise (D)

Department of Agricultural and Food Sciences, University of Bologna, 40127, Bologna, Italy.

Vincenzo Motta (V)

Department of Agricultural and Food Sciences, University of Bologna, 40127, Bologna, Italy.

Chiara Salvarani (C)

Department of Agricultural and Food Sciences, University of Bologna, 40127, Bologna, Italy.

Anisa Ribani (A)

Department of Agricultural and Food Sciences, University of Bologna, 40127, Bologna, Italy.

Samuele Bovo (S)

Department of Agricultural and Food Sciences, University of Bologna, 40127, Bologna, Italy.

Aude Simongiovanni (A)

Ajinomoto Eurolysine S.A.S., 75017, Paris, France.

Keiko Matsunaga (K)

Ajinomoto Co., Inc., Tokyo, 104-8315, Japan.

Tetsuya Takimoto (T)

Ajinomoto Co., Inc., Tokyo, 104-8315, Japan.

Makoto Bannai (M)

Ajinomoto Co., Inc., Tokyo, 104-8315, Japan.

Etienne Corrent (E)

Ajinomoto Eurolysine S.A.S., 75017, Paris, France.

Luca Fontanesi (L)

Department of Agricultural and Food Sciences, University of Bologna, 40127, Bologna, Italy.

Tristan Chalvon-Demersay (T)

Ajinomoto Eurolysine S.A.S., 75017, Paris, France.

Paolo Trevisi (P)

Department of Agricultural and Food Sciences, University of Bologna, 40127, Bologna, Italy. paolo.trevisi@unibo.it.

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Classifications MeSH