Customizable, engineered substrates for rapid screening of cellular cues.
Animals
Biocompatible Materials
/ chemistry
Cell Culture Techniques
/ instrumentation
Cell Proliferation
Cells, Cultured
Chondrocytes
/ cytology
Humans
Mice
Myocytes, Cardiac
/ cytology
Nanostructures
/ chemistry
Pluripotent Stem Cells
/ cytology
Surface Properties
Tissue Engineering
/ instrumentation
Tissue Scaffolds
/ chemistry
Journal
Biofabrication
ISSN: 1758-5090
Titre abrégé: Biofabrication
Pays: England
ID NLM: 101521964
Informations de publication
Date de publication:
07 02 2020
07 02 2020
Historique:
pubmed:
30
11
2019
medline:
15
12
2020
entrez:
30
11
2019
Statut:
epublish
Résumé
Biophysical cues robustly direct cell responses and are thus important tools for in vitro and translational biomedical applications. High throughput platforms exploring substrates with varying physical properties are therefore valuable. However, currently existing platforms are limited in throughput, the biomaterials used, the capability to segregate between different cues and the assessment of dynamic responses. Here we present a multiwell array (3 × 8) made of a substrate engineered to present topography or rigidity cues welded to a bottomless plate with a 96-well format. Both the patterns on the engineered substrate and the well plate format can be easily customized, permitting systematic and efficient screening of biophysical cues. To demonstrate the broad range of possible biophysical cues examinable, we designed and tested three multiwell arrays to influence cardiomyocyte, chondrocyte and osteoblast function. Using the multiwell array, we were able to measure different cell functionalities using analytical modalities such as live microscopy, qPCR and immunofluorescence. We observed that grooves (5 μm in size) induced less variation in contractile function of cardiomyocytes. Compared to unpatterned plastic, nanopillars with 127 nm height, 100 nm diameter and 300 nm pitch enhanced matrix deposition, chondrogenic gene expression and chondrogenic maintenance. High aspect ratio pillars with an elastic shear modulus of 16 kPa mimicking the matrix found in early stages of bone development improved osteogenic gene expression compared to stiff plastic. We envisage that our bespoke multiwell array will accelerate the discovery of relevant biophysical cues through improved throughput and variety.
Identifiants
pubmed: 31783378
doi: 10.1088/1758-5090/ab5d3f
pmc: PMC7655147
doi:
Substances chimiques
Biocompatible Materials
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
025009Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/G008868/1
Pays : United Kingdom
Organisme : Chief Scientist Office
ID : CZB/4/714
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : JF20604
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/K011235/1
Pays : United Kingdom
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