Encapsulation of curcumin loaded chitosan nanoparticle within poly (ε-caprolactone) and gelatin fiber mat for wound healing and layered dermal reconstitution.
Animals
Biocompatible Materials
/ chemistry
Capsules
Chitosan
/ chemistry
Curcumin
/ chemistry
Drug Carriers
/ chemistry
Drug Liberation
Electricity
Gelatin
/ chemistry
Male
Materials Testing
Mechanical Phenomena
Nanoparticles
/ chemistry
Polyesters
/ chemistry
Rats
Rats, Wistar
Skin
/ drug effects
Tissue Scaffolds
Wettability
Wound Healing
/ drug effects
Controlled release
Curcumin loaded chitosan nanoparticle
Gelatin
Polycaprolactone
Wound healing
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
15 Jun 2020
15 Jun 2020
Historique:
received:
06
04
2019
revised:
28
10
2019
accepted:
28
10
2019
pubmed:
24
11
2019
medline:
10
2
2021
entrez:
24
11
2019
Statut:
ppublish
Résumé
Hybrid electrospun fiber containing bioactive molecules, which offer the ability to deliver the cells into the wound bed, will help to achieve a high therapeutic effect. In this study, an electrospun polycaperlactone (PCL) and gelatin (Gela) scaffold containing curcumin loaded chitosan nanoparticle (NCs/Cur) was used to evaluate in vivo wound healing ability of the fabricated scaffolds. The electrospun hybrid scaffold seeded with human endometrial stem cells (EnSCs) showed desirable biocompatibility with the host immune system and wound healing ability in a full-thickness excisional animal model. The constructs were characterized for structural, mechanical and biochemical properties. Fourier transform infrared spectroscopy (FTIR) confirmed all typical absorption characteristics of PCL and Gela polymers as well as NCs and Cur. The results showed the perfect contact angle, wettability and degradability of hybrid fiber scaffolds with the good mechanical and structural characteristics including shape uniformity, pore size and porosity. The cell attachment and proliferation on the PCL/Gela/NCs/Cur was higher than PCL and PCL/Gela scaffolds. In term of the capability of hybrid scaffold and EnSCs in histological analysis, this novel tissue-engineered construct could be suggested as a skin substitute to repair injured skin and regenerative medicine application.
Identifiants
pubmed: 31759002
pii: S0141-8130(19)32528-0
doi: 10.1016/j.ijbiomac.2019.10.255
pii:
doi:
Substances chimiques
Biocompatible Materials
0
Capsules
0
Drug Carriers
0
Polyesters
0
polycaprolactone
24980-41-4
Gelatin
9000-70-8
Chitosan
9012-76-4
Curcumin
IT942ZTH98
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1241-1250Informations de copyright
Copyright © 2019. Published by Elsevier B.V.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare no conflicts of interest.