Impact of Donor Human Milk in the Preterm Very Low Birth Weight Gut Transcriptome Profile by Use of Exfoliated Intestinal Cells.
Caseins
/ metabolism
Cyclooxygenase 1
/ metabolism
Epithelial Cells
/ metabolism
Female
Gene Expression
Gestational Age
Humans
Infant, Newborn
Infant, Premature
Infant, Very Low Birth Weight
Intestinal Mucosa
/ cytology
Lactalbumin
/ metabolism
Male
Milk Banks
Milk, Human
/ metabolism
NADPH Oxidases
/ metabolism
Oxidative Stress
/ genetics
Principal Component Analysis
Prospective Studies
Transcriptome
/ genetics
donor milk
genetics
inflammation
intestinal cells
mother’s milk
oxidative stress
prematurity
Journal
Nutrients
ISSN: 2072-6643
Titre abrégé: Nutrients
Pays: Switzerland
ID NLM: 101521595
Informations de publication
Date de publication:
05 Nov 2019
05 Nov 2019
Historique:
received:
08
10
2019
revised:
28
10
2019
accepted:
30
10
2019
entrez:
8
11
2019
pubmed:
7
11
2019
medline:
29
4
2020
Statut:
epublish
Résumé
Own mother's milk (OMM) is the optimal nutrition for preterm infants. However, pasteurized donor human milk (DHM) is a valid alternative. We explored the differences of the transcriptome in exfoliated epithelial intestinal cells (EEIC) of preterm infants receiving full feed with OMM or DHM. The prospective observational study included preterm infants ≤ 32 weeks' gestation and/or ≤1500 g birthweight. Total RNA from EEIC were processed for genome-wide expression analysis. Principal component analysis and unsupervised hierarchical clustering analysis revealed two clustered groups corresponding to the OMM and DHM groups that showed differences in the gene expression profile in 1629 transcripts. The OMM group overexpressed lactalbumin alpha gene ( The transcriptomic analysis of EEIC showed that OMM induced a differential expression of specific genes that may contribute to a more efficient response to a pro-oxidant challenge early in the postnatal period when preterm infants are at a higher risk of oxidative stress. The use of OMM should be strongly promoted in preterm infants.
Sections du résumé
BACKGROUND
BACKGROUND
Own mother's milk (OMM) is the optimal nutrition for preterm infants. However, pasteurized donor human milk (DHM) is a valid alternative. We explored the differences of the transcriptome in exfoliated epithelial intestinal cells (EEIC) of preterm infants receiving full feed with OMM or DHM.
METHODS
METHODS
The prospective observational study included preterm infants ≤ 32 weeks' gestation and/or ≤1500 g birthweight. Total RNA from EEIC were processed for genome-wide expression analysis.
RESULTS
RESULTS
Principal component analysis and unsupervised hierarchical clustering analysis revealed two clustered groups corresponding to the OMM and DHM groups that showed differences in the gene expression profile in 1629 transcripts. The OMM group overexpressed lactalbumin alpha gene (
CONCLUSIONS
CONCLUSIONS
The transcriptomic analysis of EEIC showed that OMM induced a differential expression of specific genes that may contribute to a more efficient response to a pro-oxidant challenge early in the postnatal period when preterm infants are at a higher risk of oxidative stress. The use of OMM should be strongly promoted in preterm infants.
Identifiants
pubmed: 31694290
pii: nu11112677
doi: 10.3390/nu11112677
pmc: PMC6893464
pii:
doi:
Substances chimiques
Caseins
0
Lactalbumin
9013-90-5
Cyclooxygenase 1
EC 1.14.99.1
PTGS1 protein, human
EC 1.14.99.1
NADPH Oxidases
EC 1.6.3.-
neutrophil cytosolic factor 1
EC 1.6.3.1
Types de publication
Journal Article
Observational Study
Langues
eng
Sous-ensembles de citation
IM
Références
Matern Child Nutr. 2018 Jan;14(1):
pubmed: 28714111
J Biol Chem. 1998 Dec 4;273(49):32833-41
pubmed: 9830030
Am J Clin Nutr. 2009 Jan;89(1):210-5
pubmed: 19056604
J Pediatr Gastroenterol Nutr. 2013 Oct;57(4):535-42
pubmed: 24084373
J Pediatr Gastroenterol Nutr. 2013 May;56(5):560-8
pubmed: 23274339
Pediatr Neonatol. 2018 Dec;59(6):600-605
pubmed: 29519632
Anesth Analg. 2015 Jun;120(6):1337-51
pubmed: 25988638
J Perinatol. 2018 Jan;38(1):71-74
pubmed: 29048409
Proc Natl Acad Sci U S A. 2000 Aug 1;97(16):8841-8
pubmed: 10922044
Pediatr Rheumatol Online J. 2008 Jul 21;6:11
pubmed: 18644131
Immunity. 2012 Nov 16;37(5):771-83
pubmed: 23159225
J Proteome Res. 2015 May 1;14(5):2143-57
pubmed: 25757574
J Pediatr. 2011 Feb;158(2 Suppl):e5-7
pubmed: 21238706
Cochrane Database Syst Rev. 2018 Jun 20;6:CD002971
pubmed: 29926476
Ann N Y Acad Sci. 1999;890:301-11
pubmed: 10668435
BMB Rep. 2009 Feb 28;42(2):106-12
pubmed: 19250612
BMC Genomics. 2012 Sep 19;13:496
pubmed: 22992290
Free Radic Biol Med. 2018 Feb 1;115:179-190
pubmed: 29197632
Nihon Rinsho. 1998 Oct;56(10):2500-3
pubmed: 9796309
Breastfeed Med. 2009 Sep;4(3):137-44
pubmed: 19366315
J Agric Food Chem. 2018 Nov 14;66(45):11881-11896
pubmed: 30247884
Eur J Pharmacol. 2010 Jun 25;636(1-3):8-17
pubmed: 20371238
J Paediatr Child Health. 2013 Mar;49 Suppl 1:1-7
pubmed: 23448314
Kidney Int. 2001 Jul;60(1):14-30
pubmed: 11422732
Mol Immunol. 2007 Jul;44(15):3625-32
pubmed: 17521736
Nutrients. 2016 Aug 02;8(8):
pubmed: 27490567
Oxid Med Cell Longev. 2018 Jul 2;2018:7397659
pubmed: 30057683
Am J Clin Nutr. 2003 Jun;77(6):1537S-1543S
pubmed: 12812151
Int J Immunopathol Pharmacol. 2008 Apr-Jun;21(2):381-5
pubmed: 18547467
Immunol Rev. 2016 Jan;269(1):228-47
pubmed: 26683156
Neurosci Lett. 2005 Nov 4;388(1):7-12
pubmed: 16087294
Food Chem. 2014 May 15;151:79-85
pubmed: 24423505
Clin Perinatol. 2017 Mar;44(1):165-172
pubmed: 28159203
Pediatr Res. 2019 Jan;85(1):20-29
pubmed: 30297877
Vet Immunol Immunopathol. 2009 Mar 15;128(1-3):104-9
pubmed: 19027173
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
Kidney Int. 2007 Sep;72(5):549-56
pubmed: 17579660
FEBS Lett. 2014 Nov 17;588(22):4112-9
pubmed: 25042036
J Pediatr Gastroenterol Nutr. 2015 Jan;60(1):127-30
pubmed: 25221936
J Perinatol. 2009 Jan;29(1):57-62
pubmed: 18716628
J Infect. 2015 Jun;71 Suppl 1:S112-20
pubmed: 25934325
Front Microbiol. 2018 Jun 27;9:1376
pubmed: 29997594
Front Biosci (Elite Ed). 2011 Jun 01;3:818-29
pubmed: 21622093
Redox Biol. 2017 Aug;12:674-681
pubmed: 28395175
Ital J Pediatr. 2019 Jan 7;45(1):4
pubmed: 30616641
Sci Rep. 2016 May 16;6:25497
pubmed: 27180802
Oxid Med Cell Longev. 2016;2016:2768365
pubmed: 27403229
Toxicology. 2003 Jul 15;189(1-2):75-88
pubmed: 12821284
Clin Nutr. 2019 Aug;38(4):1844-1852
pubmed: 30093147
Sci Rep. 2014 Jun 26;4:5453
pubmed: 24965658
Biotechnol Genet Eng Rev. 2013;29:49-60
pubmed: 24568252
Am J Physiol Gastrointest Liver Physiol. 2010 May;298(5):G582-9
pubmed: 20203060