Decreased expression of femXAB genes and fnbp mediated biofilm pathways in OS-MRSA clinical isolates.
Anti-Bacterial Agents
/ pharmacology
Bacterial Proteins
/ genetics
Biofilms
Down-Regulation
Genotype
Humans
Methicillin-Resistant Staphylococcus aureus
/ drug effects
Microbial Sensitivity Tests
Multilocus Sequence Typing
Phenotype
Staphylococcal Infections
/ microbiology
Staphylococcus aureus
/ drug effects
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
05 11 2019
05 11 2019
Historique:
received:
06
10
2018
accepted:
01
10
2019
entrez:
7
11
2019
pubmed:
7
11
2019
medline:
4
11
2020
Statut:
epublish
Résumé
Methicillin-Resistant Staphylococcus aureus (MRSA) is a significant threat to human health. Additionally, biofilm forming bacteria becomes more tolerant to antibiotics and act as bacterial reservoir leading to chronic infection. In this study, we characterised the antibiotic susceptibility, biofilm production and sequence types (ST) of 74 randomly selected clinical isolates of S. aureus causing ocular infections. Antibiotic susceptibility revealed 74% of the isolates as resistant against one or two antibiotics, followed by 16% multidrug-resistant isolates (MDR), and 10% sensitive. The isolates were characterized as MRSA (n = 15), Methicillin-sensitive S. aureus (MSSA, n = 48) and oxacillin susceptible mecA positive S. aureus (OS-MRSA, n = 11) based on oxacillin susceptibility, mecA gene PCR and PBP2a agglutination test. All OS-MRSA would have been misclassified as MSSA on the basis of susceptibility test. Therefore, both phenotypic and genotypic tests should be included to prevent strain misrepresentation. In addition, in-depth studies for understanding the emerging OS-MRSA phenotype is required. The role of fem XAB gene family has been earlier reported in OS-MRSA phenotype. Sequence analysis of the fem XAB genes revealed mutations in fem × (K3R, H11N, N18H and I51V) and fem B (L410F) genes. The fem XAB genes were also found down-regulated in OS-MRSA isolates in comparison to MRSA. In OS-MRSA isolates, biofilm formation is regulated by fibronectin binding proteins A & B. Molecular typing of the isolates revealed genetic diversity. All the isolates produced biofilm, however, MRSA isolates with strong biofilm phenotype represent a worrisome situation and may even result in treatment failure.
Identifiants
pubmed: 31690794
doi: 10.1038/s41598-019-52557-z
pii: 10.1038/s41598-019-52557-z
pmc: PMC6831631
doi:
Substances chimiques
Anti-Bacterial Agents
0
Bacterial Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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