HPLC-ESI- MS/MS analysis of beet (Beta vulgaris) leaves and its beneficial properties in type 1 diabetic rats.
Animals
Antioxidants
/ metabolism
Atherosclerosis
/ blood
Beta vulgaris
/ chemistry
Blood Glucose
/ metabolism
Body Weight
/ drug effects
Chromatography, High Pressure Liquid
Cytokines
/ metabolism
Diabetes Mellitus, Experimental
/ blood
Diabetes Mellitus, Type 1
/ blood
Female
Glutathione
/ metabolism
Humans
Inflammation Mediators
/ metabolism
Lipids
/ blood
Liver
/ metabolism
Male
Malondialdehyde
/ metabolism
Molecular Docking Simulation
PPAR gamma
/ metabolism
Phenols
/ analysis
Phytochemicals
/ analysis
Phytotherapy
Plant Extracts
/ pharmacology
Plant Leaves
/ chemistry
Rats, Wistar
Spectrometry, Mass, Electrospray Ionization
Tandem Mass Spectrometry
Toxicity Tests, Acute
Beta vulgaris
C-glycosyl flavonoids
Cytokines
Molecular networking
Nuclear factor kappa B
Oxidative stress
PPAR-ɣ
Journal
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
ISSN: 1950-6007
Titre abrégé: Biomed Pharmacother
Pays: France
ID NLM: 8213295
Informations de publication
Date de publication:
Dec 2019
Dec 2019
Historique:
received:
01
08
2019
revised:
30
09
2019
accepted:
04
10
2019
pubmed:
21
10
2019
medline:
1
4
2020
entrez:
21
10
2019
Statut:
ppublish
Résumé
The phenolic profile of the leaves of Beta vulgaris subspecies vulgaris variety rubra was investigated by high-performance liquid chromatography (HPLC) coupled to electrospray ionization high resolution mass spectrometric (ESI-HRMS-MS) detection. Mass spectrometry-based molecular networking was employed to dereplicate the known compounds. Twelve known compounds, seven of which are previously undescribed as constituents in the B. vulgaris leaves were dereplicated and assigned with various levels of identification confidence. The ameliorative effects of the aqueous methanolic extract of the leaves were assessed against alloxan induced diabetic rats. It was found that the extract significantly decreased (p < 0.001) serum glucose, lipid profile, ALT, AST, TNF-α, IL-1β, IL-6, and hepatic MDA levels; and significantly increased (p < 0.001) hepatic TAO and GSH; and down-regulated the expression of hepatic NF-κB versus the untreated diabetic groups, in a dose-dependent manner. In molecular docking, all identified compounds exhibited good glide score against the PPAR-ɣ target, confirming the in vivo observed activities. In conclusion, B. vulgaris has immunomodulatory / antioxidant effects that could be helpful in slowing the progression of diabetic complications.
Identifiants
pubmed: 31629949
pii: S0753-3322(19)33807-7
doi: 10.1016/j.biopha.2019.109541
pii:
doi:
Substances chimiques
Antioxidants
0
Blood Glucose
0
Cytokines
0
Inflammation Mediators
0
Lipids
0
PPAR gamma
0
Phenols
0
Phytochemicals
0
Plant Extracts
0
Malondialdehyde
4Y8F71G49Q
Glutathione
GAN16C9B8O
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
109541Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier Masson SAS.. All rights reserved.