Adaptation of H3N2 canine influenza virus to feline cell culture.
Adaptation, Physiological
Amino Acids
/ genetics
Animals
Cats
Cell Culture Techniques
Dogs
Giant Cells
/ metabolism
Hemagglutinins
/ chemistry
Hydrogen-Ion Concentration
Influenza A Virus, H3N2 Subtype
/ genetics
Kinetics
Madin Darby Canine Kidney Cells
Models, Molecular
Mutation
/ genetics
Protein Stability
Temperature
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2019
2019
Historique:
received:
27
06
2019
accepted:
22
09
2019
entrez:
11
10
2019
pubmed:
11
10
2019
medline:
13
3
2020
Statut:
epublish
Résumé
H3N2 canine influenza viruses are prevalent in Asian and North American countries. During circulation of the viruses in dogs, these viruses are occasionally transmitted to cats. If this canine virus causes an epidemic in cats too, sporadic infections may occur in humans because of the close contact between these companion animals and humans, possibly triggering an emergence of mutant viruses with a pandemic potential. In this study, we aimed to gain an insight into the mutations responsible for inter-species transmission of H3N2 virus from dogs to cats. We found that feline CRFK cell-adapted viruses acquired several mutations in multiple genome segments. Among them, HA1-K299R, HA2-T107I, NA-L35R, and M2-W41C mutations individually increased virus growth in CRFK cells. With a combination of these mutations, virus growth further increased not only in CRFK cells but also in other feline fcwf-4 cells. Both HA1-K299R and HA2-T107I mutations increased thermal resistance of the viruses. In addition, HA2-T107I increased the pH requirement for membrane fusion. These findings suggest that the mutations, especially the two HA mutations, identified in this study, might be responsible for adaptation of H3N2 canine influenza viruses in cats.
Identifiants
pubmed: 31600274
doi: 10.1371/journal.pone.0223507
pii: PONE-D-19-18114
pmc: PMC6786582
doi:
Substances chimiques
Amino Acids
0
Hemagglutinins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0223507Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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