Steady-state kinetics of the tungsten containing aldehyde: ferredoxin oxidoreductases from the hyperthermophilic archaeon Pyrococcus furiosus.
Aldehyde Oxidoreductases
/ antagonists & inhibitors
Aldehydes
/ metabolism
Archaeal Proteins
/ antagonists & inhibitors
Enzyme Inhibitors
Glyceraldehyde 3-Phosphate
/ chemistry
Glyceraldehyde-3-Phosphate Dehydrogenases
/ antagonists & inhibitors
Hydrogen-Ion Concentration
Kinetics
Oxidation-Reduction
Pyrococcus furiosus
/ enzymology
Sodium Chloride
Substrate Specificity
Temperature
Tungsten
/ metabolism
Aldehyde ferredoxin oxidoreductase
Glyceraldehyde-3-phosphate oxidoreductase
Pyrococcus furiosus
Steady state kinetics
Tungsten enzyme
Journal
Journal of biotechnology
ISSN: 1873-4863
Titre abrégé: J Biotechnol
Pays: Netherlands
ID NLM: 8411927
Informations de publication
Date de publication:
20 Dec 2019
20 Dec 2019
Historique:
received:
02
08
2019
revised:
22
09
2019
accepted:
03
10
2019
pubmed:
8
10
2019
medline:
9
4
2020
entrez:
8
10
2019
Statut:
ppublish
Résumé
The tungsten containing Aldehyde:ferredoxin oxidoreductases (AOR) offer interesting opportunities for biocatalytic approaches towards aldehyde oxidation and carboxylic acid reduction. The hyperthermophilic archaeon Pyrococcus furiosus encodes five different AOR family members: glyceraldehyde-3-phosphate oxidoreductase (GAPOR), aldehyde oxidoreductase (AOR), and formaldehyde oxidoreductase (FOR), WOR4 and WOR5. GAPOR functions as a glycolytic enzyme and is highly specific for the substrate glyceraldehyde-3-phosphate (GAP). AOR, FOR and WOR5 have a broad substrate spectrum, and for WOR4 no substrate has been identified to date. As ambiguous kinetic parameters have been reported for different AOR family enzymes the steady state kinetics under different physiologically relevant conditions was explored. The GAPOR substrate GAP was found to degrade at 60 °C by non-enzymatic elimination of the phosphate group to methylglyoxal with a half-life t
Identifiants
pubmed: 31589889
pii: S0168-1656(19)30885-5
doi: 10.1016/j.jbiotec.2019.10.005
pii:
doi:
Substances chimiques
Aldehydes
0
Archaeal Proteins
0
Enzyme Inhibitors
0
Glyceraldehyde 3-Phosphate
142-10-9
Sodium Chloride
451W47IQ8X
Aldehyde Oxidoreductases
EC 1.2.-
Glyceraldehyde-3-Phosphate Dehydrogenases
EC 1.2.1.-
aldehyde ferredoxin oxidoreductase
EC 1.2.7.-
formaldehyde ferredoxin oxidoreductase
EC 1.2.7.-
glyceraldehyde-3-phosphate ferredoxin oxidoreductase
EC 1.2.7.-
Tungsten
V9306CXO6G
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
142-148Informations de copyright
Copyright © 2019. Published by Elsevier B.V.