Evaluation of CpG-ODN-adjuvanted polyanhydride-based intranasal influenza nanovaccine in pigs.


Journal

Veterinary microbiology
ISSN: 1873-2542
Titre abrégé: Vet Microbiol
Pays: Netherlands
ID NLM: 7705469

Informations de publication

Date de publication:
Oct 2019
Historique:
received: 20 06 2019
revised: 27 08 2019
accepted: 27 08 2019
entrez: 6 10 2019
pubmed: 6 10 2019
medline: 29 1 2020
Statut: ppublish

Résumé

Influenza results in significant economic loss in the swine industry each year. A broadly protective swine influenza vaccine would have the dual benefit of protecting pigs from influenza A viruses (IAVs) and limiting their possible zoonotic transmission to humans. In this study, we developed polyanhydride nanoparticles-based swine influenza vaccine (KAg + CpG-nanovaccine) co-encapsulating inacticated/killed soluble antigen (KAg) and Toll-like receptor (TLR)-9 agonist (CpG-ODN). The immunogenicity and protective efficacy of KAg + CpG-nanovaccine was compared with KAg vaccine containing five-times greater quantity of antigens following heterologous virus challenge. Prime-boost intranasally delivered KAg + CpG-nanovaccine induced significantly higher levels of cross-reactive antigen-specific IgA antibody responses in the nasal cavity, greater lymphoproliferative response in peripheral blood mononuclear cells (PBMCs), and higher IFN-γ secretion during antigen-induced recall responses of PBMCs and tracheobronchial lymph nodes cells compared to those immunized with KAg alone. Importantly, KAg + CpG-nanovaccine provided better protective efficacy through a significant reduction in influenza-induced fever, 16-fold reduction of nasal virus shedding and 80-fold reduction in lung virus titers compared to those immunized with soluble KAg. Our results indicated that CpG-ODN-adjuvanted polyanhydride nanovaccine can induce higher mucosal antibody and cellular immune responses in pigs; and provide better protection as compared with intranasally delivered soluble KAg.

Identifiants

pubmed: 31585639
pii: S0378-1135(19)30731-X
doi: 10.1016/j.vetmic.2019.108401
pii:
doi:

Substances chimiques

Adjuvants, Immunologic 0
Antibodies, Viral 0
Antigens, Viral 0
CPG-oligonucleotide 0
Immunoglobulin A 0
Influenza Vaccines 0
Oligodeoxyribonucleotides 0
Polyanhydrides 0
Vaccines, Inactivated 0
Interferon-gamma 82115-62-6

Types de publication

Journal Article Randomized Controlled Trial, Veterinary

Langues

eng

Sous-ensembles de citation

IM

Pagination

108401

Informations de copyright

Copyright © 2019 Elsevier B.V. All rights reserved.

Auteurs

S Dhakal (S)

Food Animal Health Research Program (FAHRP), Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, OH, 44691, USA.

S Ghimire (S)

Food Animal Health Research Program (FAHRP), Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, OH, 44691, USA.

S Renu (S)

Food Animal Health Research Program (FAHRP), Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, OH, 44691, USA.

K A Ross (KA)

Department of Chemical and Biological Engineering, Iowa State University, Ames, IA 50011, USA.

Y S Lakshmanappa (YS)

Food Animal Health Research Program (FAHRP), Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, OH, 44691, USA.

B T Hogshead (BT)

Food Animal Health Research Program (FAHRP), Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, OH, 44691, USA.

P Bernardo (P)

Food Animal Health Research Program (FAHRP), Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, OH, 44691, USA.

C W Lee (CW)

Food Animal Health Research Program (FAHRP), Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, OH, 44691, USA.

M J Wannemuehler (MJ)

Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, IA, USA.

B Narasimhan (B)

Department of Chemical and Biological Engineering, Iowa State University, Ames, IA 50011, USA.

G J Renukaradhya (GJ)

Food Animal Health Research Program (FAHRP), Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, OH, 44691, USA. Electronic address: gourapura.1@osu.edu.

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Classifications MeSH