Evaluation of CpG-ODN-adjuvanted polyanhydride-based intranasal influenza nanovaccine in pigs.
Adjuvants, Immunologic
Administration, Intranasal
Animals
Antibodies, Viral
Antigens, Viral
/ immunology
Female
Immunity, Mucosal
Immunoglobulin A
/ immunology
Influenza Vaccines
/ immunology
Interferon-gamma
/ metabolism
Leukocytes, Mononuclear
Male
Nanostructures
Oligodeoxyribonucleotides
/ immunology
Orthomyxoviridae Infections
/ prevention & control
Polyanhydrides
Swine
Swine Diseases
/ prevention & control
Vaccines, Inactivated
/ immunology
CpG-ODN
Intranasal vaccine
Pigs
Polyanhydride nanoparticles
Swine influenza A virus
Journal
Veterinary microbiology
ISSN: 1873-2542
Titre abrégé: Vet Microbiol
Pays: Netherlands
ID NLM: 7705469
Informations de publication
Date de publication:
Oct 2019
Oct 2019
Historique:
received:
20
06
2019
revised:
27
08
2019
accepted:
27
08
2019
entrez:
6
10
2019
pubmed:
6
10
2019
medline:
29
1
2020
Statut:
ppublish
Résumé
Influenza results in significant economic loss in the swine industry each year. A broadly protective swine influenza vaccine would have the dual benefit of protecting pigs from influenza A viruses (IAVs) and limiting their possible zoonotic transmission to humans. In this study, we developed polyanhydride nanoparticles-based swine influenza vaccine (KAg + CpG-nanovaccine) co-encapsulating inacticated/killed soluble antigen (KAg) and Toll-like receptor (TLR)-9 agonist (CpG-ODN). The immunogenicity and protective efficacy of KAg + CpG-nanovaccine was compared with KAg vaccine containing five-times greater quantity of antigens following heterologous virus challenge. Prime-boost intranasally delivered KAg + CpG-nanovaccine induced significantly higher levels of cross-reactive antigen-specific IgA antibody responses in the nasal cavity, greater lymphoproliferative response in peripheral blood mononuclear cells (PBMCs), and higher IFN-γ secretion during antigen-induced recall responses of PBMCs and tracheobronchial lymph nodes cells compared to those immunized with KAg alone. Importantly, KAg + CpG-nanovaccine provided better protective efficacy through a significant reduction in influenza-induced fever, 16-fold reduction of nasal virus shedding and 80-fold reduction in lung virus titers compared to those immunized with soluble KAg. Our results indicated that CpG-ODN-adjuvanted polyanhydride nanovaccine can induce higher mucosal antibody and cellular immune responses in pigs; and provide better protection as compared with intranasally delivered soluble KAg.
Identifiants
pubmed: 31585639
pii: S0378-1135(19)30731-X
doi: 10.1016/j.vetmic.2019.108401
pii:
doi:
Substances chimiques
Adjuvants, Immunologic
0
Antibodies, Viral
0
Antigens, Viral
0
CPG-oligonucleotide
0
Immunoglobulin A
0
Influenza Vaccines
0
Oligodeoxyribonucleotides
0
Polyanhydrides
0
Vaccines, Inactivated
0
Interferon-gamma
82115-62-6
Types de publication
Journal Article
Randomized Controlled Trial, Veterinary
Langues
eng
Sous-ensembles de citation
IM
Pagination
108401Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.