The effect of allogenic human Wharton's jelly stem cells seeded onto acellular dermal matrix in healing of rat burn wounds.
Wharton's jelly stem cells
acellular dermal matrix
burn
healing
wound
Journal
Journal of cosmetic dermatology
ISSN: 1473-2165
Titre abrégé: J Cosmet Dermatol
Pays: England
ID NLM: 101130964
Informations de publication
Date de publication:
Apr 2020
Apr 2020
Historique:
received:
24
04
2019
revised:
20
07
2019
accepted:
23
07
2019
pubmed:
27
9
2019
medline:
20
1
2021
entrez:
27
9
2019
Statut:
ppublish
Résumé
Various methods were introduced to overcome the autograft shortage in burn wound care, including cell transplantation and tissue engineering. To evaluate the healing effect of allogenic human Wharton's jelly stem cells (hWJSCs) seeded onto acellular dermal matrix (ADM) in rat burn injuries. Human Wharton's jelly stem cells provided from umbilical cord tissue were characterized before transplantation, and the growth kinetic was determined. Skin samples from cosmetic surgeries were used for preparation of ADM. Forty male Sprague Dawley rats were randomly divided into 4 equal groups. Third-degree burn was induced for all animals by exposing to hot water using a 2 cm ring for 10 seconds. Group 1 was burned rats that did not receive any treatment. After burn injury, the second group received silver sulfadiazine (SSD), the third group was treated just by using ADM, and the fourth group received 2 × 10 Human Wharton's jelly stem cells were characterized to be spindle shape and positive for osteogenic and adipogenic induction and for mesenchymal markers but lacked hematopoietic markers. Population doubling time (PDT) was 40.1 hours with an increasing growth trend until day 6th. Macro- and microscopically, the healing was mild in ADM group and moderate in ADM + hWJSCs group after 21 days. Allogenic hWJSCs seeded onto ADM improved the healing process in burn wounds denoting to their therapeutic and anti-inflammatory effects in burn wounds that can be added to the literature.
Sections du résumé
BACKGROUND
BACKGROUND
Various methods were introduced to overcome the autograft shortage in burn wound care, including cell transplantation and tissue engineering.
AIMS
OBJECTIVE
To evaluate the healing effect of allogenic human Wharton's jelly stem cells (hWJSCs) seeded onto acellular dermal matrix (ADM) in rat burn injuries.
PATIENTS AND METHODS
METHODS
Human Wharton's jelly stem cells provided from umbilical cord tissue were characterized before transplantation, and the growth kinetic was determined. Skin samples from cosmetic surgeries were used for preparation of ADM. Forty male Sprague Dawley rats were randomly divided into 4 equal groups. Third-degree burn was induced for all animals by exposing to hot water using a 2 cm ring for 10 seconds. Group 1 was burned rats that did not receive any treatment. After burn injury, the second group received silver sulfadiazine (SSD), the third group was treated just by using ADM, and the fourth group received 2 × 10
RESULTS
RESULTS
Human Wharton's jelly stem cells were characterized to be spindle shape and positive for osteogenic and adipogenic induction and for mesenchymal markers but lacked hematopoietic markers. Population doubling time (PDT) was 40.1 hours with an increasing growth trend until day 6th. Macro- and microscopically, the healing was mild in ADM group and moderate in ADM + hWJSCs group after 21 days.
CONCLUSION
CONCLUSIONS
Allogenic hWJSCs seeded onto ADM improved the healing process in burn wounds denoting to their therapeutic and anti-inflammatory effects in burn wounds that can be added to the literature.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
995-1001Subventions
Organisme : National Institute for Medical Research Development of Iran Ministry of Health, Treatment and Medical Education
ID : 963474
Informations de copyright
© 2019 Wiley Periodicals, Inc.
Références
World Health Organization. 2016 Burns fact sheet worldwide. 2017;2:9. http://www.who.int/mediacentre/factsheets/fs365/en Accessed 6 March 2018.
Franck CL, Senegaglia AC, Leite L, de Moura S, Francisco NF, Ribas Filho JM. Influence of adipose tissue-derived stem cells on the burn wound healing process. Stem Cells Int. 2019;2019:2340725.
Rowan MP, Cancio LC, Elster EA, et al. Burn wound healing and treatment: review and advancements. Crit Care. 2015;19:243.
Halim AS, Khoo TL, Yussof M. Biologic and synthetic skin substitutes: an overview. Indian J Plast Surg. 2010;43:S23-S28.
Middelkoop E, van den Bogaerdt AJ, Lamme EN, Hoekstra MJ, Brandsma K, Ulrich MM. Porcine wound models for skin substitution and burn treatment. Biomaterials. 2004;25:1559-1567.
Mohtasham Amiri Z, Tanideh N, Seddighi A, et al. The effect of lithospermum officinale, silver sulfadiazine and alpha ointments in healing of burn wound injuries in rat. World J Plast Surg. 2017;6:313-318.
Sedighi A, Mehrabani D, Shirazi R. Histopathological evaluation of the healing effects of human amniotic membrane transplantation in third-degree burn wound injuries. Comp Clin Pathol. 2016;25:381-385.
Mehrabani D, Rabiee M, Tamadon A, et al. The growth kinetic, differentiation properties, karyotyping, and characterization of adipose tissue-derived stem cells in hamster. Comp Clin Pathol. 2016;25:1017-1022.
Iravani K, Sobhanmanesh A, Ashraf MJ, Hashemi SB, Mehrabani D, Zare S. The healing effect of conditioned media and bone marrow-derived stem cells in laryngotracheal stenosis: a comparison in experimental dog model. World J Plast Surg. 2017;6:190-197.
Mehrabani D, Khodakaram-Tafti A, Shaterzadeh-Yazdi H, Zamiri B, Omidi M. Comparison of the regenerative effect of adipose-derived stem cells, fibrin glue scaffold, and autologous bone graft in experimental mandibular defect in rabbit. Dent Traumatol. 2018;34:413-420.
Mehrabani D, Mahdiyar P, Torabi K, et al. Growth kinetics and characterization of human dental pulp stem cells: comparison between third molar and first premolar teeth. J Clin Exp Dent. 2017;9:e172-e177.
Mehrabani D, Mehrabani G, Zare S, Manafi A. Adipose-derived stem cells (ADSC) and aesthetic surgery: a mini review. World J Plast Surg. 2013;2:65-70.
Huang L, Burd A. An update review of stem cell applications in burns and wound care. Indian J Plast Surg. 2012;45:229-236.
Kong CM, Lin HD, Biswas A, Bongso A, Fong CY. Manufacturing of human Wharton's jelly stem cells for clinical use: selection of serum is important. Cytotherapy. 2019;21(4):483-495. [Epub ahead of print].
Han Y, Sun T, Han Y, et al. Human umbilical cord mesenchymal stem cells implantation accelerates cutaneous wound healing in diabetic rats via the Wnt signaling pathway. Eur J Med Res. 2019;24:10.
Khodakaram-Tafti A, Mehrabani D, Shaterzadeh-Yazdi H, Zamiri B, Omidi M. Tissue engineering in maxillary bone defects. World J Plast Surg. 2018;7:3-11.
Zhang CP, Fu XB. Therapeutic potential of stem cells in skin repair and regeneration. Chin J Traumatol. 2008;11:209-221.
Namnoum JD. Expander/implant reconstruction with alloderm: recent experience. Plast Reconstr Surg. 2009;124:387-394.
Lai C, Song G, Zhao B, et al. Preparation and characterization of human scar acellular dermal matrix. J Biomater Sci Polym Ed. 2019;30:769-784. [Epub ahead of print]).
Wainwright DJ. Use of an acellular allograft dermal matrix (AlloDerm) in the management of full-thickness burns. Burns. 1995;21:243-248.
Shumakov VI, Onishchenko NA, Rasulov MF, Krasheninnikov ME, Zaidenov VA. Mesenchymal bone marrow stem cells more effectively stimulate regeneration of deep burn wounds than embryonic fibroblasts. Bull Exp Biol Med. 2003;136:192-195.
Imam RA, Rizk AA. Efficacy of erythropoietin pretreated mesenchymal stem cells in murine burn wound healing: possible in vivo transdifferentiation into keratinocytes. Folia Morphol (Warsz). 2019. [Epub ahead of print].
FDA. proposed approach to regulation of cellular and tissue-based products. http://www.fda.gov/downloads/Biologi-casBloodVaccines/GuidanceComplianceRe-gulatoryInformation/Guidances/Tissue/UCM-062601. Accessed on 26 July, 2011.
Karimi H, Soudmand A, Orouji Z, Taghiabadi E, Mousavi SJ. Burn wound healing with injection of adipose-derived stem cells: a mouse model study. Ann Burns Fire Disasters. 2014;27:44-49.
Chang YW, Wu YC, Huang SH, Wang H, Kuo YR, Lee SS. Autologous and not allogeneic adipose-derived stem cells improve acute burn wound healing. PLoS One. 2018;13:e0197744.
Francis E, Kearney L, Clover J. The effects of stem cells on burn wounds: a review. Int J Burns Trauma. 2019;9(1):1-12. eCollection.
Orbay H, Takami Y, Hyakusoku H, Mizuno H. Acellular dermal matrix seeded with adipose-derived stem cells as a subcutaneous implant. Aesth Plast Surg. 2011;35:756-763.
Yang Y, Zhang W, Li Y, Fang G, Zhang K. Scalded skin of rat treated by using fibrin glue combined with allogeneic bone marrow mesenchymal stem cells. Ann Dermatol. 2014;26:289-295.
Nambu M, Ishihara M, Nakamura S, et al. Enhanced healing of mitomycin C-treated wounds in rats using inbred adipose tissue-derived stromal cells within an atelocollagen matrix. Wound Repair Regen. 2007;15:505-510.
Motamed S, Taghiabadi E, Molaei H, et al. Cell-based skin substitutes accelerate regeneration of extensive burn wounds in rats. Am J Surg. 2017;214:762-769.
Yan G, Sun H, Wang F, et al. Topical application of hPDGF-A-modified porcine BMSC and keratinocytes loaded on acellular HAM promotes the healing of combined radiation-wound skin injury in minipigs. Int J Radiat Biol. 2011;87:591-600.
Ghieh F, Jurjus R, Ibrahim A, et al. The use of stem cells in burn wound healing: a review. Biomed Res Int. 2015;2015:684084.
Foubert P, Barillas S, Gonzalez AD, et al. Uncultured adipose-derived regenerative cells (ADRCs) seeded in collagen scaffold improves dermal regeneration, enhancing early vascularization and structural organization following thermal burns. Burns. 2015;41:1504-1516.
Chen Y-W, Scutaru TT, Ghetu N, et al. The effects of adipose-derived stem cell-differentiated adipocytes on skin burn wound healing in rats. J Burn Care Res. 2017;38:1-10.
Singer DD, Singer AJ, Gordon C, Brink P. The effects of rat mesenchymal stem cells on injury progression in a rat model. Acad Emerg Med. 2013;20:398-402.
Liu P, Deng Z, Han S, et al. Tissue-engineered skin containing mesenchymal stem cells improves burn wounds. Artif Organs. 2008;32:925-931.
Cruz FF, Rocco P. Stem-cell extracellular vesicles and lung repair. Stem Cell Investig. 2017;4:78.
Liu L, Yu Y, Hou Y, et al. Human umbilical cord mesenchymal stem cells transplantation promotes cutaneous wound healing of severe burned rats. PLoS One. 2014;9:e88348.
Clover AJP, Kumar AHS, Isakson M, et al. Allogeneic mesenchymal stem cells, but not culture modified monocytes, improve burn wound healing. Burns. 2015;41:548-557.