Evaluation of Acridine Orange Staining for a Semi-Automated Urinalysis Microscopic Examination at the Point-of-Care.
acridine orange
cell classification
fluorescence
point-of-care
urinalysis
urinary tract infection
Journal
Diagnostics (Basel, Switzerland)
ISSN: 2075-4418
Titre abrégé: Diagnostics (Basel)
Pays: Switzerland
ID NLM: 101658402
Informations de publication
Date de publication:
18 Sep 2019
18 Sep 2019
Historique:
received:
29
08
2019
revised:
11
09
2019
accepted:
16
09
2019
entrez:
22
9
2019
pubmed:
22
9
2019
medline:
22
9
2019
Statut:
epublish
Résumé
A urinary tract infection (UTI) can be diagnosed via urinalysis, consisting of a dipstick test and manual microscopic examination. Point-of-care (POC) image-based systems have been designed to automate the microscopic examination for low-volume laboratories or low-resource clinics. In this pilot study, acridine orange (AO) was evaluated as a fluorescence-based contrast agent to aid in detecting and enumerating urine sediment specific for diagnosing a UTI. Acridine orange staining of epithelial cells, leukocytes, and bacteria provided sufficient contrast to successfully implement image segmentation techniques, which enabled the extraction of classifiable morphologic features. Surface area bounded by each cell border was used to differentiate the sediment; epithelial cells were larger than 500μm
Identifiants
pubmed: 31540364
pii: diagnostics9030122
doi: 10.3390/diagnostics9030122
pmc: PMC6787640
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Arkansas Biosciences Institute
ID : N/A
Organisme : Arkansas Women's Giving Circle
ID : N/A
Références
BMJ Open. 2015 May 18;5(5):e006857
pubmed: 25986635
J Clin Microbiol. 1982 Apr;15(4):562-6
pubmed: 6175656
J Pediatr. 1993 Jan;122(1):22-5
pubmed: 8419610
Clin Chim Acta. 2007 Sep;384(1-2):28-34
pubmed: 17604012
Biochem Med (Zagreb). 2012;22(3):276-82
pubmed: 23092060
J Clin Microbiol. 2016 Dec;54(12):2975-2981
pubmed: 27682127
Invest Urol. 1976 Mar;13(5):331-8
pubmed: 1254426
J Biomed Opt. 2017 Mar 1;22(3):35001
pubmed: 28253379
Clin Infect Dis. 2004 Apr 15;38(8):1150-8
pubmed: 15095222
Infect Control Hosp Epidemiol. 2017 Apr;38(4):455-460
pubmed: 28052781
Clin Chim Acta. 2016 May 1;456:31-35
pubmed: 26921459
Trends Biotechnol. 2015 Nov;33(11):692-705
pubmed: 26463722
N Engl J Med. 2003 Jun 5;348(23):2330-8
pubmed: 12788998
Pract Lab Med. 2016 Mar 11;5:14-20
pubmed: 28856199
Lab Chip. 2014 Aug 21;14(16):3029-36
pubmed: 24955810
Am J Kidney Dis. 2016 Jun;67(6):954-64
pubmed: 26806004
Clin Chem. 1974 Apr;20(4):436-9
pubmed: 4818195
Pediatrics. 2011 Sep;128(3):595-610
pubmed: 21873693
J Clin Microbiol. 1981 Aug;14(2):201-5
pubmed: 6168652
J Clin Lab Anal. 2008;22(4):262-70
pubmed: 18623125
Am J Med. 2002 Jul 8;113 Suppl 1A:5S-13S
pubmed: 12113866
Cell. 2014 Jan 30;156(3):456-68
pubmed: 24485454
Hypertens Pregnancy. 2005;24(2):117-23
pubmed: 16036396
J Clin Lab Anal. 2018 Feb;32(2):null
pubmed: 28464263
Scand J Clin Lab Invest. 2011 Feb;71(1):30-7
pubmed: 21091139
Clin Nephrol. 2001 May;55(5):384-92
pubmed: 11393384
PLoS Med. 2012;9(9):e1001306
pubmed: 22973183
J Pediatr. 2018 Nov;202:238-244.e1
pubmed: 30172436
Am J Clin Pathol. 2000 May;113(5):709-13
pubmed: 10800404
Cytometry A. 2014 Aug;85(8):729-37
pubmed: 24953340
Lab Chip. 2018 Jul 10;18(14):2111-2123
pubmed: 29926053
Clin Chim Acta. 2012 Feb 18;413(3-4):478-82
pubmed: 22120731
J Urol. 1980 Apr;123(4):478-85
pubmed: 7365880
Am Fam Physician. 2005 Mar 15;71(6):1153-62
pubmed: 15791892
J Infect Dis. 1994 Apr;169(4):839-46
pubmed: 8133099
Clin Chem Lab Med. 2015 Nov;53 Suppl 2:s1509-11
pubmed: 26057217
Clin Lab. 2012;58(9-10):979-85
pubmed: 23163114
IEEE J Transl Eng Health Med. 2017 Dec 15;6:2800111
pubmed: 29333352
Cancer Res. 1977 Aug;37(8 Pt 2):2806-12
pubmed: 141325
J Biomed Opt. 2015 Oct;20(10):105010
pubmed: 26502228
Clin Biochem. 2012 Jul;45(10-11):856-8
pubmed: 22548911
J Clin Microbiol. 2018 Jul 26;56(8):
pubmed: 29769277
J Glob Infect Dis. 2009 Jan;1(1):57-63
pubmed: 20300389