Comparative metabolomics of MCF-7 breast cancer cells using different extraction solvents assessed by mass spectroscopy.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
11 09 2019
11 09 2019
Historique:
received:
21
05
2019
accepted:
27
08
2019
entrez:
13
9
2019
pubmed:
13
9
2019
medline:
5
11
2020
Statut:
epublish
Résumé
Metabolic profiling of cancer cells can play a vital role in revealing the molecular bases of cancer development and progression. In this study, gas chromatography coupled with mass spectrometry (GC-MS) was employed for the determination of signatures found in ER+/PR+ breast cancer cells derived from MCF-7 using different extraction solvents including: A, formic acid in water; B, ammonium hydroxide in water; C, ethyl acetate; D, methanol: water (1:1, v/v); and E, acetonitrile: water (1:1, v/v). The greatest extraction rate and diversity of metabolites occurs with extraction solvents A and E. Extraction solvent D showed moderate extraction efficiency, whereas extraction solvent B and C showed inferior metabolite diversity. Metabolite set enrichment analysis (MSEA) results showed energy production pathways to be key in MCF-7 cell lines. This study showed that mass spectrometry could identify key metabolites associated with cancers. The highest enriched pathways were related to energy production as well as Warburg effect pathways, which may shed light on how energy metabolism has been hijacked to encourage tumour progression and eventually metastasis in breast cancer.
Identifiants
pubmed: 31511569
doi: 10.1038/s41598-019-49509-y
pii: 10.1038/s41598-019-49509-y
pmc: PMC6739366
doi:
Substances chimiques
Acetonitriles
0
Formates
0
Solvents
0
Water
059QF0KO0R
formic acid
0YIW783RG1
Methanol
Y4S76JWI15
acetonitrile
Z072SB282N
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
13126Références
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