The fine-tuning of endoplasmic reticulum stress response and autophagy activation during trophoblast syncytialization.
Journal
Cell death & disease
ISSN: 2041-4889
Titre abrégé: Cell Death Dis
Pays: England
ID NLM: 101524092
Informations de publication
Date de publication:
09 09 2019
09 09 2019
Historique:
received:
30
04
2019
accepted:
09
08
2019
revised:
19
07
2019
entrez:
11
9
2019
pubmed:
11
9
2019
medline:
1
9
2020
Statut:
epublish
Résumé
The syncytiotrophoblast (STB) is a multinuclear layer forming the outer surface of the fetal part of the placenta deriving from villous cytotrophoblastic cell (vCTB) fusion and differentiation. This syncytialization process is characterized by morphological and biochemical alterations of the trophoblast, which probably require removal of pre-existing structures and proteins to maintain cell homeostasis and survival. Interestingly, autophagy, which allows degradation and recycling of cellular components, was shown to be activated in syncytiotrophoblast. Here we examined the involvement of endoplasmic reticulum stress (ERS) response in autophagy activation during vCTB syncytialization. We first demonstrated the activation of ERS response and autophagy during the time course of trophoblastic cell fusion and differentiation. Alteration of autophagy activation in vCTB by chemical treatments or Beclin-1 expression modulation leads to a decrease in trophoblastic syncytialization. Furthermore, ERS response inhibition by chemical treatment or siRNA strategy leads to a default in syncytialization, associated with alteration of autophagy markers and cell survival. From these data, we suggest that ERS response, by fine regulation of autophagy activation, may serve as an adaptive mechanism to promote cell survival during trophoblastic syncytialization.
Identifiants
pubmed: 31501418
doi: 10.1038/s41419-019-1905-6
pii: 10.1038/s41419-019-1905-6
pmc: PMC6733854
doi:
Substances chimiques
Beclin-1
0
RNA, Small Interfering
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
651Références
J Matern Fetal Neonatal Med. 2013 Mar;26(4):339-46
pubmed: 23039021
J Biochem. 2013 Jan;153(1):13-9
pubmed: 23172303
J Mol Biol. 2009 Sep 18;392(2):301-18
pubmed: 19616006
Hum Reprod Update. 2004 Nov-Dec;10(6):487-96
pubmed: 15333590
PLoS One. 2017 Nov 6;12(11):e0186909
pubmed: 29107968
Electron Microsc Rev. 1991;4(1):129-78
pubmed: 1873486
JCI Insight. 2016 Dec 22;1(21):e86654
pubmed: 28018968
Cell Mol Life Sci. 2018 May;75(10):1839-1855
pubmed: 29080089
Am J Obstet Gynecol. 1997 Jul;177(1):57-65
pubmed: 9240583
Am J Obstet Gynecol. 2015 Oct;213(4 Suppl):S9.e1, S9-11
pubmed: 26428507
PLoS One. 2012;7(8):e40596
pubmed: 22912664
Mol Cell. 2010 Oct 22;40(2):280-93
pubmed: 20965422
Philos Trans R Soc Lond B Biol Sci. 2015 Mar 5;370(1663):20140066
pubmed: 25602070
Clin Exp Reprod Med. 2015 Mar;42(1):1-7
pubmed: 25874167
Placenta. 2017 Sep;57:163-169
pubmed: 28864006
Placenta. 2014 Dec;35(12):974-80
pubmed: 25465707
Dev Cell. 2007 Sep;13(3):351-64
pubmed: 17765679
J Cell Physiol. 2017 Sep;232(9):2581-2589
pubmed: 27770612
Placenta. 2009 Mar;30 Suppl A:S49-54
pubmed: 19027159
J Reprod Immunol. 2017 Sep;123:17-23
pubmed: 28869810
PLoS One. 2010 Mar 08;5(3):e9575
pubmed: 20221394
Autophagy. 2013 Dec;9(12):2140-53
pubmed: 24113155
Early Pregnancy. 1995 Dec;1(4):263-9
pubmed: 9363258
J Pathol. 2014 Oct;234(2):262-76
pubmed: 24931423
Int J Mol Sci. 2016 Apr 28;17(5):
pubmed: 27136533
Placenta. 2010 Feb;31(2):89-96
pubmed: 20080300
J Thromb Haemost. 2014 Jun;12(6):910-20
pubmed: 24656088
Cell Death Differ. 2004 Jan;11(1):90-8
pubmed: 12970672
PLoS One. 2013 Dec 13;8(12):e83475
pubmed: 24349516
Elife. 2016 Jul 20;5:
pubmed: 27435960
Mol Reprod Dev. 2008 Jan;75(1):175-83
pubmed: 17546632
Hum Reprod Update. 2000 Sep-Oct;6(5):485-94
pubmed: 11045879
Cell Death Differ. 2015 Mar;22(3):367-76
pubmed: 25257169
Biochem J. 2014 Sep 1;462(2):267-77
pubmed: 24865278