XANES and EXAFS of dilute solutions of transition metals at XFELs.
X-ray absorption spectroscopy
X-ray free electron lasers
X-ray near-edge spectroscopy
extended X-ray absorption fine structure
Journal
Journal of synchrotron radiation
ISSN: 1600-5775
Titre abrégé: J Synchrotron Radiat
Pays: United States
ID NLM: 9888878
Informations de publication
Date de publication:
01 Sep 2019
01 Sep 2019
Historique:
received:
18
10
2018
accepted:
23
05
2019
entrez:
7
9
2019
pubmed:
7
9
2019
medline:
7
9
2019
Statut:
ppublish
Résumé
This work has demonstrated that X-ray absorption spectroscopy (XAS), both Mn XANES and EXAFS, of solutions with millimolar concentrations of metal is possible using the femtosecond X-ray pulses from XFELs. Mn XAS data were collected using two different sample delivery methods, a Rayleigh jet and a drop-on-demand setup, with varying concentrations of Mn. Here, a new method for normalization of XAS spectra based on solvent scattering that is compatible with data collection from a highly variable pulsed source is described. The measured XANES and EXAFS spectra of such dilute solution samples are in good agreement with data collected at synchrotron sources using traditional scanning protocols. The procedures described here will enable XFEL-based XAS on dilute biological samples, especially metalloproteins, with low sample consumption. Details of the experimental setup and data analysis methods used in this XANES and EXAFS study are presented. This method will also benefit XAS performed at high-repetition-rate XFELs such as the European XFEL, LCLS-II and LCLS-II-HE.
Identifiants
pubmed: 31490163
pii: S1600577519007550
doi: 10.1107/S1600577519007550
pmc: PMC6730626
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1716-1724Subventions
Organisme : Vetenskapsrådet
ID : 2017-00356
Organisme : NIH HHS
ID : GM126289
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM126289
Pays : United States
Organisme : NIGMS NIH HHS
ID : R56 GM055302
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM110501
Pays : United States
Organisme : NIH HHS
ID : GM055302
Pays : United States
Organisme : NIGMS NIH HHS
ID : F32 GM116423
Pays : United States
Organisme : U.S. Department of Energy, Office of Basic Energy Sciences
ID : DE-AC02-76SF00515
Organisme : Vetenskapsrådet
ID : 2016-05183
Organisme : NIGMS NIH HHS
ID : R01 GM055302
Pays : United States
Organisme : NIH HHS
ID : GM116423-02
Pays : United States
Organisme : NIH HHS
ID : GM110501
Pays : United States
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