Misshapen coordinates protrusion restriction and actomyosin contractility during collective cell migration.

Actin Cytoskeleton / metabolism Actomyosin / genetics Algorithms Animals Animals, Genetically Modified Cell Movement / genetics Drosophila Proteins / genetics Drosophila melanogaster / genetics Female Gene Expression Regulation, Developmental Microfilament Proteins / genetics Models, Genetic Oogenesis / genetics Protein Serine-Threonine Kinases / genetics RNA Interference

Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
02 09 2019
Historique:
received: 04 06 2018
accepted: 19 07 2019
entrez: 4 9 2019
pubmed: 4 9 2019
medline: 31 12 2019
Statut: epublish

Résumé

Collective cell migration is involved in development, wound healing and metastasis. In the Drosophila ovary, border cells (BC) form a small cluster that migrates collectively through the egg chamber. To achieve directed motility, the BC cluster coordinates the formation of protrusions in its leader cell and contractility at the rear. Restricting protrusions to leader cells requires the actin and plasma membrane linker Moesin. Herein, we show that the Ste20-like kinase Misshapen phosphorylates Moesin in vitro and in BC. Depletion of Misshapen disrupts protrusion restriction, thereby allowing other cells within the cluster to protrude. In addition, we show that Misshapen is critical to generate contractile forces both at the rear of the cluster and at the base of protrusions. Together, our results indicate that Misshapen is a key regulator of BC migration as it coordinates two independent pathways that restrict protrusion formation to the leader cells and induces contractile forces.

Identifiants

DOI: 10.1038/s41467-019-11963-7 PMID: 31477736 PMC: PMC6718686
pubmed: 31477736
doi: 10.1038/s41467-019-11963-7
pii: 10.1038/s41467-019-11963-7
pmc: PMC6718686
doi:

Substances chimiques

Drosophila Proteins 0
Microfilament Proteins 0
moesin 144131-77-1
Actomyosin 9013-26-7
msn protein, Drosophila EC 2.7.1.-
Protein Serine-Threonine Kinases EC 2.7.11.1

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

3940

Subventions

Organisme : CIHR
ID : MOP-148560
Pays : Canada
Organisme : CIHR
ID : MOP-142374
Pays : Canada
Organisme : CIHR
ID : MOP-133683
Pays : Canada

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Auteurs

Cédric Plutoni (C)

Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, Montréal, QC, Canada.

Sarah Keil (S)

Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, Montréal, QC, Canada.

Carlos Zeledon (C)

Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, Montréal, QC, Canada.

Lara Elis Alberici Delsin (LEA)

Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, Montréal, QC, Canada.

Barbara Decelle (B)

Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, Montréal, QC, Canada.

Philippe P Roux (PP)

Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, Montréal, QC, Canada.
Department of Pathology and Cell Biology, Faculty of Medicine, Université de Montréal, Montréal, QC, Canada.
ORCID: 0000-0002-5962-0250

Sébastien Carréno (S)

Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, Montréal, QC, Canada.
Department of Pathology and Cell Biology, Faculty of Medicine, Université de Montréal, Montréal, QC, Canada.
ORCID: 0000-0002-5839-2102

Gregory Emery (G)

Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, Montréal, QC, Canada. gregory.emery@umontreal.ca.
Department of Pathology and Cell Biology, Faculty of Medicine, Université de Montréal, Montréal, QC, Canada. gregory.emery@umontreal.ca.
ORCID: 0000-0002-6874-6875

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Classifications MeSH

Recherchemedicale.com › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Cytosquelette › Cytosquelette d'actine › Misshapen coordinates protrusion restriction...
Recherchemedicale.com › Acides aminés, peptides et protéines › Protéines › Protéines contractiles › Protéines du muscle › Actomyosine › Misshapen coordinates protrusion restriction...
Recherchemedicale.com › Sciences de l'information › Méthodologies informatiques › Algorithmes › Misshapen coordinates protrusion restriction...
Recherchemedicale.com › Eucaryotes › Animaux › Misshapen coordinates protrusion restriction...
Recherchemedicale.com › Stades d'organismes › Organismes génétiquement modifiés › Animal génétiquement modifié › Misshapen coordinates protrusion restriction...
Recherchemedicale.com › Phénomènes physiologiques › Mouvement cellulaire › Misshapen coordinates protrusion restriction...
Recherchemedicale.com › Acides aminés, peptides et protéines › Protéines › Protéines d'arthropode › Protéines d'insecte › Protéines de Drosophila › Misshapen coordinates protrusion restriction...
Recherchemedicale.com › Eucaryotes › Animaux › Invertébrés › Arthropodes › Insectes › Pterygota › Neoptera › Holometabola › Diptera › Drosophilidae › Drosophila › Drosophila melanogaster › Misshapen coordinates protrusion restriction...
Recherchemedicale.com › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes au cours du développement › Misshapen coordinates protrusion restriction...
Recherchemedicale.com › Acides aminés, peptides et protéines › Protéines › Protéines du cytosquelette › Protéines des microfilaments › Misshapen coordinates protrusion restriction...
Recherchemedicale.com › Techniques d'investigation › Modèles théoriques › Modèles biologiques › Modèles génétiques › Misshapen coordinates protrusion restriction...
Recherchemedicale.com › Phénomènes physiologiques des appareils urinaire et reproducteur › Phénomènes physiologiques de la reproduction › Reproduction › Gamétogenèse › Ovogenèse › Misshapen coordinates protrusion restriction...
Recherchemedicale.com › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protein-Serine-Threonine Kinases › Misshapen coordinates protrusion restriction...
Recherchemedicale.com › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Interférence par ARN › Misshapen coordinates protrusion restriction...