No magnesium is needed for binding of the stimulator of interferon genes to cyclic dinucleotides.
3′,3′-c-di-GMP
CDN
STING
cGAS
crystal structure
Journal
Acta crystallographica. Section F, Structural biology communications
ISSN: 2053-230X
Titre abrégé: Acta Crystallogr F Struct Biol Commun
Pays: United States
ID NLM: 101620319
Informations de publication
Date de publication:
01 Sep 2019
01 Sep 2019
Historique:
received:
09
07
2019
accepted:
06
08
2019
entrez:
3
9
2019
pubmed:
3
9
2019
medline:
27
2
2020
Statut:
ppublish
Résumé
Stimulator of interferon genes (STING) binds cyclic dinucleotides (CDNs), which induce a large conformational change of the protein. The structural basis of activation of STING by CDNs is rather well understood. Unliganded STING forms an open dimer that undergoes a large conformational change (∼10 Å) to a closed conformation upon the binding of a CDN molecule. This event activates downstream effectors of STING and subsequently leads to activation of the type 1 interferon response. However, a previously solved structure of STING with 3',3'-c-di-GMP shows Mg atoms mediating the interaction of STING with this CDN. Here, it is shown that no Mg atoms are needed for this interaction; in fact, magnesium can in some cases obstruct the binding of a CDN to STING.
Identifiants
pubmed: 31475926
pii: S2053230X19010999
doi: 10.1107/S2053230X19010999
pmc: PMC6718146
doi:
Substances chimiques
Membrane Proteins
0
STING1 protein, human
0
Cyclic GMP
H2D2X058MU
Magnesium
I38ZP9992A
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
593-598Subventions
Organisme : Akademie Věd České Republiky
ID : RVO:61388963
Références
Acta Crystallogr D Biol Crystallogr. 2012 Apr;68(Pt 4):425-30
pubmed: 22505262
Mol Cell. 2013 Jul 25;51(2):226-35
pubmed: 23747010
J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674
pubmed: 19461840
Nature. 2019 Mar;567(7748):394-398
pubmed: 30842653
Nat Immunol. 2003 May;4(5):491-6
pubmed: 12692549
Sci Transl Med. 2015 Apr 15;7(283):283ra52
pubmed: 25877890
Cell Rep. 2015 May 19;11(7):1018-30
pubmed: 25959818
Proc Natl Acad Sci U S A. 2015 Jul 21;112(29):8947-52
pubmed: 26150511
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):235-42
pubmed: 21460441
Nat Rev Immunol. 2015 Dec;15(12):760-70
pubmed: 26603901
Nat Struct Mol Biol. 2012 Jun 24;19(7):722-4
pubmed: 22728658
Biochim Biophys Acta. 2015 May;1854(5):333-40
pubmed: 25644306
Nat Struct Mol Biol. 2012 Jun 24;19(7):728-30
pubmed: 22728659
Mol Cell. 2012 Jun 29;46(6):735-45
pubmed: 22705373
Nat Immunol. 2016 Sep 20;17(10):1142-9
pubmed: 27648547
Arch Virol. 2017 Jul;162(7):2091-2096
pubmed: 28357511
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21
pubmed: 20124702
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):125-32
pubmed: 20124692
Nucleic Acids Res. 2017 Jan 25;45(2):987-1004
pubmed: 27923930
Acta Crystallogr D Biol Crystallogr. 2010 Dec;66(Pt 12):1351-7
pubmed: 21123876
Nat Rev Mol Cell Biol. 2019 May;20(5):266
pubmed: 30872810
Nature. 2019 Mar;567(7748):389-393
pubmed: 30842659