Formation of Virus-Like Particles of the Dengue Virus Serotype 2 Expressed in Silkworm Larvae.
Animals
Bombyx
/ growth & development
Capsid Proteins
/ biosynthesis
Dengue Virus
/ genetics
Fat Body
/ metabolism
Gene Expression
Genetic Vectors
Hemolymph
/ metabolism
Heparin
/ metabolism
Larva
/ metabolism
Nucleopolyhedroviruses
/ genetics
Protein Sorting Signals
/ genetics
Serogroup
Viral Envelope Proteins
/ biosynthesis
Viral Structural Proteins
/ biosynthesis
Virion
/ genetics
Bacmid
Capsid
Dengue virus
Envelope
Premembrane
Silkworm
Journal
Molecular biotechnology
ISSN: 1559-0305
Titre abrégé: Mol Biotechnol
Pays: Switzerland
ID NLM: 9423533
Informations de publication
Date de publication:
Nov 2019
Nov 2019
Historique:
pubmed:
2
9
2019
medline:
27
2
2020
entrez:
2
9
2019
Statut:
ppublish
Résumé
To explore virus-like particles formation of dengue virus serotype type 2 (DENV-2) structural proteins of, C, prM, E were expressed in silkworm larvae using recombinant Bombyx mori nucleopolyhedroviruses (BmNPV). Each recombinant BmNPV bacmid coding the 2C-prM-E polypeptide and E protein fused with the signal peptide of bombyxin from B. mori was injected into silkworm larvae. The expressed proteins were collected from hemolymph and fat body, and purified using affinity chromatography. E protein was observed at 55 kDa. The DENV virus-like particles (DENV-LPs) with a diameter approximately 35 nm was observed using transmission electron microscopy (TEM) and immunogold-labelling TEM analysis. The binding of each partially purified proteins to heparin, one of receptors for DENV was confirmed. DENV-LPs were secreted in silkworm larval hemolymph even still low amount, but the E protein and heparin binding function were confirmed.
Identifiants
pubmed: 31473916
doi: 10.1007/s12033-019-00210-5
pii: 10.1007/s12033-019-00210-5
doi:
Substances chimiques
Capsid Proteins
0
Protein Sorting Signals
0
Viral Envelope Proteins
0
Viral Structural Proteins
0
Heparin
9005-49-6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
852-859Subventions
Organisme : Japan Society for the Promotion of Science
ID : 16K14900
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