Giant cells and osteoclasts present in bone grafted with nacre differ by nuclear cytometry evaluated by texture analysis.
Animal Shells
/ chemistry
Animals
Bone Substitutes
/ analysis
Bone Transplantation
/ instrumentation
Bone-Anchored Prosthesis
Cell Nucleus
/ chemistry
Foreign-Body Reaction
/ pathology
Giant Cells
/ pathology
Histological Techniques
/ methods
Image Cytometry
/ methods
Nacre
/ chemistry
Osteoclasts
/ pathology
Pinctada
Sheep
Journal
Journal of materials science. Materials in medicine
ISSN: 1573-4838
Titre abrégé: J Mater Sci Mater Med
Pays: United States
ID NLM: 9013087
Informations de publication
Date de publication:
29 Aug 2019
29 Aug 2019
Historique:
received:
13
02
2019
accepted:
26
07
2019
entrez:
31
8
2019
pubmed:
31
8
2019
medline:
6
2
2020
Statut:
epublish
Résumé
Nacre (mother of pearl) is a natural biomaterial used to prepare orthopedic devices. We have implanted screws and plates made with nacre in five sheeps. Bone were harvested after two months and embedded in poly(methyl methacrylate). Blocks were saws and the thick slabs were grinded, polished and surface stained. Sections were photographed at an ×1000 magnification. Giant cells were found in contact with nacre in eroded areas and true osteoclasts were found at distance in the neighboring bone in Howship lacunae. A texture analysis of the nuclei of giant cells and osteoclasts was done using the run-length method of the MaZda freeware. The size of the nuclei was reduced in osteoclast and their mean gray level appeared reduced. Texture analysis revealed that chromatin had a completely different pattern in giant cells when compared to osteoclasts. Giant cells had a fine repartition of the chromatin with large clear areas around prominent nucleoli. On the contrary, osteoclast nuclei had chromatin blocks evenly dispersed in the nuclei. This reflects the different origin of these cells expressing different functions.
Identifiants
pubmed: 31468139
doi: 10.1007/s10856-019-6293-z
pii: 10.1007/s10856-019-6293-z
doi:
Substances chimiques
Bone Substitutes
0
Nacre
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
100Références
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