Mixed monolayer decorated SPR sensing surface for thrombin detection.
3,3′ Dithiodipropionic acid di (N-hydroxysuccinimide ester) DSP:6-mercapto-1-hexanol (MCH)
Biological recognition element immobilization
Biosensor
Gold surfaces
Mixed self-assembled monolayers (mSAMs)
Surface plasmon resonance (SPR)
Journal
Journal of pharmaceutical and biomedical analysis
ISSN: 1873-264X
Titre abrégé: J Pharm Biomed Anal
Pays: England
ID NLM: 8309336
Informations de publication
Date de publication:
30 Nov 2019
30 Nov 2019
Historique:
received:
29
12
2018
revised:
16
08
2019
accepted:
18
08
2019
pubmed:
28
8
2019
medline:
11
3
2020
entrez:
28
8
2019
Statut:
ppublish
Résumé
The development of surface plasmon resonance (SPR) based immunosensor for thrombin detection was aimed. For this purpose, 3,3' Dithiodipropionic acid di (N-hydroxysuccinimide ester) (DSP):6-mercapto-1-hexanol (MCH) mixed self-assembled monolayers (mSAMs) were formed on gold surfaces for immobilization of anti-thrombin antibody. The performance of the immunosensor was determined against the target protein thrombin at various concentrations using flow cell coupled SPR. The linear detection range of the immunosensor was 30.0-100.0 nM with an R
Identifiants
pubmed: 31454662
pii: S0731-7085(18)32924-8
doi: 10.1016/j.jpba.2019.112822
pii:
doi:
Substances chimiques
Antibodies
0
Thrombin
EC 3.4.21.5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
112822Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.