Luminescent Nanosensors for Ratiometric Monitoring of Three-Dimensional Oxygen Gradients in Laboratory and Clinical Pseudomonas aeruginosa Biofilms.


Journal

Applied and environmental microbiology
ISSN: 1098-5336
Titre abrégé: Appl Environ Microbiol
Pays: United States
ID NLM: 7605801

Informations de publication

Date de publication:
15 10 2019
Historique:
received: 17 05 2019
accepted: 09 08 2019
pubmed: 20 8 2019
medline: 24 7 2020
entrez: 18 8 2019
Statut: epublish

Résumé

Bacterial biofilms can form persistent infections on wounds and implanted medical devices and are associated with many chronic diseases, such as cystic fibrosis. These infections are medically difficult to treat, as biofilms are more resistant to antibiotic attack than their planktonic counterparts. An understanding of the spatial and temporal variation in the metabolism of biofilms is a critical component toward improved biofilm treatments. To this end, we developed oxygen-sensitive luminescent nanosensors to measure three-dimensional (3D) oxygen gradients, an application of which is demonstrated here with

Identifiants

pubmed: 31420335
pii: AEM.01116-19
doi: 10.1128/AEM.01116-19
pmc: PMC6805078
pii:
doi:

Substances chimiques

Oxygen S88TT14065

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : NINDS NIH HHS
ID : P30 NS048154
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS086839
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR001082
Pays : United States

Informations de copyright

Copyright © 2019 American Society for Microbiology.

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Auteurs

Megan P Jewell (MP)

Chemical and Biological Engineering Department, Colorado School of Mines, Golden, Colorado, USA.

Anne A Galyean (AA)

Chemical and Biological Engineering Department, Colorado School of Mines, Golden, Colorado, USA.

J Kirk Harris (J)

Department of Pediatrics, School of Medicine, University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA.

Edith T Zemanick (ET)

Department of Pediatrics, School of Medicine, University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA.
Children's Hospital Colorado, Aurora, Colorado, USA.

Kevin J Cash (KJ)

Chemical and Biological Engineering Department, Colorado School of Mines, Golden, Colorado, USA kcash@mines.edu.

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Classifications MeSH