High-Fat Feeding in Time-Dependent Manner Affects Metabolic Routes Leading to Nervonic Acid Synthesis in NAFLD.
Animals
Biomarkers
Diet, High-Fat
/ adverse effects
Disease Models, Animal
Fatty Acid Transport Proteins
/ genetics
Fatty Acids, Monounsaturated
/ metabolism
Gene Expression Regulation
Lipid Metabolism
Lipids
/ blood
Lipogenesis
Liver
/ metabolism
Male
Non-alcoholic Fatty Liver Disease
/ etiology
Rats
Diacylglycerol
desaturation
diet and dietary lipids
elongation
fatty acid metabolism
fatty acids
lipogenesis
liver
nervonic acid
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
05 Aug 2019
05 Aug 2019
Historique:
received:
09
07
2019
revised:
29
07
2019
accepted:
01
08
2019
entrez:
8
8
2019
pubmed:
8
8
2019
medline:
8
1
2020
Statut:
epublish
Résumé
Nonalcoholic fatty liver disease (NAFLD) is characterized by excessive lipid accumulation in the liver. The disturbances in the fatty acid composition of stored lipids are more important than the lipid species itself, which may influence the overall effect caused by these molecules. Thus, uncovering time-dependent changes in the fatty acid composition of accumulated lipid fractions after a high fat diet seems to be a new marker of NAFLD occurrence. The experiments were conducted on high fat fed Wistar rats. The blood and liver samples were collected at the end of each experimental week and used to assess the content of lipid fractions and their fatty acid composition by gas liquid chromatography. The expression of proteins from lipid metabolism pathways and of fatty acid exporting proteins were detected by Western blotting. In the same high fat feeding period, decreased de novo lipogenesis, increased β-oxidation and lipid efflux were demonstrated. The observed effects may be the first liver protective mechanisms against lipotoxicity. Nevertheless, such effects were still not sufficient to prevent the liver from proinflammatory lipid accumulation. Moreover, the changes in liver metabolic pathways caused the plasma nervonic acid concentration in sphingomyelin to decrease simultaneously with NAFLD development, which may be a steatosis occurrence prognostic marker.
Identifiants
pubmed: 31387306
pii: ijms20153829
doi: 10.3390/ijms20153829
pmc: PMC6695804
pii:
doi:
Substances chimiques
Biomarkers
0
Fatty Acid Transport Proteins
0
Fatty Acids, Monounsaturated
0
Lipids
0
nervonic acid
91OQS788BE
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Medical University of Białystok
ID : N/ST/ZB/17/010/1118
Déclaration de conflit d'intérêts
The authors declare no competing interests.
Références
Proc Natl Acad Sci U S A. 2003 Mar 18;100(6):3077-82
pubmed: 12629214
J Biol Chem. 1957 May;226(1):497-509
pubmed: 13428781
Hepatology. 2004 Dec;40(6):1387-95
pubmed: 15565570
Hepatology. 2005 Jun;41(6):1313-21
pubmed: 15915461
Int Heart J. 2005 Nov;46(6):975-85
pubmed: 16394593
Int J Mol Med. 2008 Jul;22(1):133-7
pubmed: 18575786
J Gastroenterol Hepatol. 2009 Jul;24(7):1284-8
pubmed: 19476560
Gastroenterology. 2012 Apr;142(4):711-725.e6
pubmed: 22326434
Lipids. 2012 Oct;47(10):941-50
pubmed: 22923371
World J Gastroenterol. 2012 Nov 7;18(41):5839-47
pubmed: 23139599
Acta Physiol (Oxf). 2013 Feb;207(2):346-57
pubmed: 23140342
Prog Lipid Res. 2013 Jan;52(1):165-74
pubmed: 23178552
Hepatology. 2014 Feb;59(2):713-23
pubmed: 23929732
J Oleo Sci. 2014;63(5):527-37
pubmed: 24770479
Prostaglandins Leukot Essent Fatty Acids. 2015 Apr;95:31-40
pubmed: 25559239
J Lipid Res. 2015 Mar;56(3):722-36
pubmed: 25598080
PLoS One. 2015 Jan 30;10(1):e0116858
pubmed: 25635851
Nutrients. 2015 Jun 19;7(6):4995-5019
pubmed: 26102213
Niger J Clin Pract. 2015 Nov-Dec;18(6):703-12
pubmed: 26289505
Lipids Health Dis. 2016 Feb 01;15:20
pubmed: 26832365
Dig Dis Sci. 2016 May;61(5):1282-93
pubmed: 26856717
Lipids Health Dis. 2016 Feb 11;15:27
pubmed: 26868515
Environ Toxicol Pharmacol. 2016 Jun;44:140-50
pubmed: 27179602
Nutr Metab (Lond). 2016 Aug 09;13:51
pubmed: 27512407
Curr Obes Rep. 2016 Dec;5(4):405-412
pubmed: 27687811
Nutrients. 2016 Oct 29;8(11):
pubmed: 27801862
Lipids. 1988 Dec;23(12):1114-8
pubmed: 3226227
Diabetes Res Clin Pract. 1994 Apr;23(3):169-77
pubmed: 7924877
J Lipid Res. 1998 Nov;39(11):2161-71
pubmed: 9799802