Transcriptional profiling aligned with in situ expression image analysis reveals mosaically expressed molecular markers for GABA neuron sub-groups in the ventral tegmental area.
aversion
dopamine
limbic
midbrain
reward
Journal
The European journal of neuroscience
ISSN: 1460-9568
Titre abrégé: Eur J Neurosci
Pays: France
ID NLM: 8918110
Informations de publication
Date de publication:
12 2019
12 2019
Historique:
received:
26
04
2019
revised:
12
07
2019
accepted:
19
07
2019
pubmed:
3
8
2019
medline:
4
9
2020
entrez:
3
8
2019
Statut:
ppublish
Résumé
γ-Aminobutyric acid (GABA) neurons in the ventral tegmental area (VTA) provide local inhibitory control of dopamine neuron activity and send long-range projections to several target regions including the nucleus accumbens. They play diverse roles in reward and aversion, suggesting that they be comprised of several functionally distinct sub-groups, but our understanding of this diversity has been limited by a lack of molecular markers that might provide genetic entry points for cell type-specific investigations. To address this, we conducted transcriptional profiling of GABA neurons and dopamine neurons using immunoprecipitation of tagged polyribosomes (RiboTag) and RNAseq. First, we directly compared these two transcriptomes in order to obtain a list of genes enriched in GABA neurons compared with dopamine neurons. Next, we created a novel bioinformatic approach, that used the PANTHER (Protein ANalysis THrough Evolutionary Relationships) gene ontology database and VTA gene expression data from the Allen Mouse Brain Atlas, from which we obtained 6 candidate genes: Cbln4, Rxfp3, Rora, Gpr101, Trh and Nrp2. As a final step, we verified the selective expression of these candidate genes in sub-groups of GABA neurons in the VTA (and neighbouring substantia nigra pars compacta) using immunolabelling. Taken together, our study provides a valuable toolbox for the future investigation of GABA neuron sub-groups in the VTA.
Identifiants
pubmed: 31374129
doi: 10.1111/ejn.14534
pmc: PMC6972656
doi:
Substances chimiques
gamma-Aminobutyric Acid
56-12-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3732-3749Subventions
Organisme : Medical Research Council
ID : MC_U120085816
Pays : United Kingdom
Informations de copyright
© 2019 The Authors. European Journal of Neuroscience published by Federation of European Neuroscience Societies and John Wiley & Sons Ltd.
Références
Synapse. 2007 Feb;61(2):87-95
pubmed: 17117419
Neuron. 2012 Mar 22;73(6):1184-94
pubmed: 22445345
Trends Neurosci. 2015 Apr;38(4):217-25
pubmed: 25637939
J Endocrinol. 2015 Mar;224(3):R139-59
pubmed: 25563352
Neuron. 2012 Mar 22;73(6):1173-83
pubmed: 22445344
N Engl J Med. 2014 Dec 18;371(25):2363-74
pubmed: 25470569
Science. 2008 Jul 4;321(5885):53-7
pubmed: 18599766
J Comp Neurol. 2010 Oct 1;518(19):4016-45
pubmed: 20737598
Bioinformatics. 2015 Jan 15;31(2):166-9
pubmed: 25260700
Nat Neurosci. 2019 Jan;22(1):106-119
pubmed: 30559475
Neuron. 2015 Nov 18;88(4):774-91
pubmed: 26549332
Hum Mol Genet. 2006 Oct 15;15 Spec No 2:R271-7
pubmed: 16987893
Development. 2016 Feb 1;143(3):516-29
pubmed: 26718003
Nature. 1980 Jun 12;285(5765):476-8
pubmed: 6105617
J Comp Neurol. 1984 Feb 1;222(4):543-59
pubmed: 6365985
Sci Rep. 2015 Nov 10;5:16176
pubmed: 26553597
J Neurosci. 2001 May 1;21(9):3126-34
pubmed: 11312297
Nature. 2012 Dec 20;492(7429):452-6
pubmed: 23178810
Nature. 2012 Jan 18;482(7383):85-8
pubmed: 22258508
J Comp Neurol. 2019 Apr 1;527(5):916-941
pubmed: 30393861
Neurobiol Aging. 2015 Feb;36(2):1057-71
pubmed: 25534236
Neuroscience. 2007 Jan 5;144(1):165-90
pubmed: 17071007
FEBS J. 2007 Jul;274(14):3568-3577
pubmed: 17565601
Nature. 2007 Jan 11;445(7124):168-76
pubmed: 17151600
Nucleic Acids Res. 2017 Jan 4;45(D1):D183-D189
pubmed: 27899595
Proc Natl Acad Sci U S A. 2009 Mar 24;106(12):4894-9
pubmed: 19261850
Neuroscience. 2008 Apr 9;152(4):1024-31
pubmed: 18355970
Front Neuroanat. 2010 Dec 29;4:150
pubmed: 21228905
Eur J Neurosci. 2019 Dec;50(11):3732-3749
pubmed: 31374129
J Comp Neurol. 2000 May 22;421(1):107-35
pubmed: 10813775
J Comp Neurol. 2014 Oct 1;522(14):3308-34
pubmed: 24715505
Exp Brain Res. 1989;74(1):149-62
pubmed: 2564343
Synapse. 2009 Oct;63(10):895-906
pubmed: 19582784
Eur J Neurosci. 2012 Nov;36(10):3322-3332
pubmed: 22925150
Neuropeptides. 1988 Apr;11(3):95-100
pubmed: 3133572
Genome Biol. 2014;15(12):550
pubmed: 25516281
Neuron. 2019 Feb 20;101(4):748-761.e5
pubmed: 30638902
Nat Protoc. 2013 Aug;8(8):1551-66
pubmed: 23868073
Eur J Neurosci. 2013 May;37(10):1573-83
pubmed: 23534961
J Neurosci. 2018 Nov 21;38(47):10080-10092
pubmed: 30282729
Neuropsychopharmacology. 2019 Jan;44(2):372-380
pubmed: 29875446
Cell. 2016 Oct 6;167(2):566-580.e19
pubmed: 27716510
Cell. 2016 Mar 24;165(1):207-219
pubmed: 26949184
J Mol Endocrinol. 2016 Aug;57(2):97-111
pubmed: 27282544
J Neurochem. 2012 Jun;121(5):717-29
pubmed: 22220752
Nature. 2014 Jan 16;505(7483):318-26
pubmed: 24429630
Brain Res. 1988 Jul 5;455(1):88-98
pubmed: 3046712
Cell. 2017 Oct 19;171(3):522-539.e20
pubmed: 28942923
Neuron. 2004 Aug 19;43(4):527-37
pubmed: 15312651
Acta Physiol (Oxf). 2013 Apr;207(4):616-27
pubmed: 23297792
J Neurosci. 2009 Oct 7;29(40):12542-57
pubmed: 19812329
Br J Pharmacol. 2017 May;174(10):1034-1048
pubmed: 27774604
Brain Res. 1992 Aug 7;587(2):203-10
pubmed: 1356063
Nat Struct Mol Biol. 2005 May;12(5):441-8
pubmed: 15821743
Eur J Neurosci. 2011 Apr;33(8):1447-61
pubmed: 21410790
Exp Brain Res. 1994;99(1):34-42
pubmed: 7925794
Cell Tissue Res. 2004 Oct;318(1):53-61
pubmed: 15340832
Rev Endocr Metab Disord. 2016 Dec;17(4):545-558
pubmed: 27515033
J Comp Neurol. 2004 Feb 2;469(2):170-84
pubmed: 14694532
Genome Biol. 2013 Apr 25;14(4):R36
pubmed: 23618408
eNeuro. 2018 Nov 16;5(5):
pubmed: 30456293
Nature. 2009 Dec 24;462(7276):1065-9
pubmed: 20010807
Dev Biol. 1999 Jun 15;210(2):305-21
pubmed: 10357893
Mech Dev. 1998 Jan;70(1-2):147-53
pubmed: 9510031
Glia. 2007 Jan 15;55(2):165-77
pubmed: 17078026
J Neurosci. 2001 Mar 1;21(5):1452-63
pubmed: 11222635
Proc Natl Acad Sci U S A. 2009 Aug 18;106(33):13939-44
pubmed: 19666516
Neuron. 2000 Jan;25(1):43-56
pubmed: 10707971
Nature. 2012 Nov 8;491(7423):212-7
pubmed: 23064228
Neuroscience. 1996 Nov;75(2):523-33
pubmed: 8931015
Neuron. 2016 Jul 20;91(2):260-92
pubmed: 27477017
Mol Cell Biol. 1999 Feb;19(2):1558-68
pubmed: 9891089