Low-cost solution for rodent home-cage behaviour monitoring.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2019
2019
Historique:
received:
01
05
2019
accepted:
22
07
2019
entrez:
3
8
2019
pubmed:
3
8
2019
medline:
7
3
2020
Statut:
epublish
Résumé
In the current research on measuring complex behaviours/phenotyping in rodents, most of the experimental design requires the experimenter to remove the animal from its home-cage environment and place it in an unfamiliar apparatus (novel environment). This interaction may influence behaviour, general well-being, and the metabolism of the animal, affecting the phenotypic outcome even if the data collection method is automated. Most of the commercially available solutions for home-cage monitoring are expensive and usually lack the flexibility to be incorporated with existing home-cages. Here we present a low-cost solution for monitoring home-cage behaviour of rodents that can be easily incorporated to practically any available rodent home-cage. To demonstrate the use of our system, we reliably predict the sleep/wake state of mice in their home-cage using only video. We validate these results using hippocampal local field potential (LFP) and electromyography (EMG) data. Our approach provides a low-cost flexible methodology for high-throughput studies of sleep, circadian rhythm and rodent behaviour with minimal experimenter interference.
Identifiants
pubmed: 31374097
doi: 10.1371/journal.pone.0220751
pii: PONE-D-19-12280
pmc: PMC6677321
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0220751Commentaires et corrections
Type : ErratumIn
Déclaration de conflit d'intérêts
Some of the authors (EBC, MHM, and RJS) have a financial interest in the company “NeuroCage Systems Inc.” that is developing a product “Homecage Monitoring System” whose initial design was inspired by the results presented in this work. Some of the authors (SS, EBC, MHM, and RJS) have a patent application that includes some of the functionality presented in this work. Pending patent for SYSTEMS AND METHODS OF HOMECAGE MONITORING; patent application # 62/857,879 (US) and 3,045,478 (Canada). There are no further products in development, or marketed products to declare. This does not alter our adherence to all the PLOS ONE policies on sharing data and materials.
Références
Cell. 1999 Aug 20;98(4):437-51
pubmed: 10481909
Physiol Behav. 2001 Aug;73(5):873-86
pubmed: 11566220
Nature. 2002 Jun 13;417(6890):684-7
pubmed: 12066153
Lab Anim. 2002 Oct;36(4):378-95
pubmed: 12396281
J Neurosci Methods. 2004 Feb 15;133(1-2):173-9
pubmed: 14757358
Nat Neurosci. 2004 May;7(5):462-6
pubmed: 15114359
Nat Rev Neurosci. 2004 Nov;5(11):874-85
pubmed: 15496865
Genes Brain Behav. 2006 Aug;5(6):458-66
pubmed: 16923150
Physiol Genomics. 2007 Jan 17;28(2):232-8
pubmed: 16985007
Proc Natl Acad Sci U S A. 2007 Feb 6;104(6):1983-8
pubmed: 17261803
IEEE Trans Biomed Eng. 2007 Feb;54(2):225-33
pubmed: 17278579
Nat Rev Neurosci. 2007 Aug;8(8):577-81
pubmed: 17643087
Neuron. 2007 Oct 4;56(1):43-57
pubmed: 17920014
Neuron. 2008 Mar 13;57(5):634-60
pubmed: 18341986
Behav Brain Res. 2008 Jun 26;190(1):74-84
pubmed: 18342378
Proc Biol Sci. 2008 Aug 22;275(1645):1865-74
pubmed: 18460434
Mt Sinai J Med. 2010 Jan-Feb;77(1):69-81
pubmed: 20101721
Nat Commun. 2010 Sep 07;1:68
pubmed: 20842193
J Neurosci Methods. 2011 Mar 30;196(2):276-80
pubmed: 21256865
Nat Methods. 2011 Sep 11;8(10):871-8
pubmed: 21909102
J Biol Rhythms. 2012 Feb;27(1):48-58
pubmed: 22306973
Sleep. 2012 Mar 01;35(3):433-42
pubmed: 22379250
J Neurosci Methods. 2012 Jul 30;209(1):74-8
pubmed: 22698663
Nat Methods. 2012 Jun 28;9(7):690-6
pubmed: 22743774
Nat Commun. 2013;4:2018
pubmed: 23771126
Nat Neurosci. 2013 Oct;16(10):1426-35
pubmed: 23974708
PLoS One. 2013 Dec 06;8(12):e83171
pubmed: 24349451
Behav Res Methods. 2015 Mar;47(1):235-50
pubmed: 24706080
Nat Neurosci. 2014 May;17(5):661-3
pubmed: 24728269
Nat Methods. 2014 Jul;11(7):743-8
pubmed: 24880877
Drug Discov Today Technol. 2006 Summer;3(2):231-7
pubmed: 24980412
Curr Protoc Mouse Biol. 2015 Sep 01;5(3):271-281
pubmed: 26331760
Proc Natl Acad Sci U S A. 2015 Sep 22;112(38):E5351-60
pubmed: 26354123
Behav Brain Res. 2016 Feb 15;299:141-6
pubmed: 26592165
Nat Commun. 2016 Jun 13;7:11611
pubmed: 27291514
Front Behav Neurosci. 2016 Jun 10;10:106
pubmed: 27375446
Wellcome Open Res. 2016 Nov 15;1:2
pubmed: 27976750
Exp Brain Res. 2017 Jun;235(6):1919-1932
pubmed: 28315945
J Neurosci Methods. 2018 Apr 15;300:37-47
pubmed: 28456660
Hippocampus. 2017 Sep;27(9):951-958
pubmed: 28686806
Sci Rep. 2017 Sep 5;7(1):10560
pubmed: 28874680
PLoS One. 2017 Sep 6;12(9):e0181068
pubmed: 28877172
Nat Commun. 2017 Oct 30;8(1):1196
pubmed: 29084948
J Alzheimers Dis. 2018;61(1):283-293
pubmed: 29154281
Neurophotonics. 2018 Apr;5(2):025005
pubmed: 29651448
Nat Neurosci. 2018 Sep;21(9):1281-1289
pubmed: 30127430
Brain Plast. 2018 Dec 12;4(1):17-52
pubmed: 30564545