4-Bromo-1,8-naphthalimide derivatives as fluorogenic substrates for live cell imaging of glutathione S-transferase (GST) activity.
Journal
Talanta
ISSN: 1873-3573
Titre abrégé: Talanta
Pays: Netherlands
ID NLM: 2984816R
Informations de publication
Date de publication:
01 Nov 2019
01 Nov 2019
Historique:
received:
24
04
2019
revised:
13
06
2019
accepted:
14
06
2019
entrez:
31
7
2019
pubmed:
31
7
2019
medline:
18
12
2019
Statut:
ppublish
Résumé
Fluorogenic substrates are used to visualize the activity of cancer-associated enzymes and to interpret biological events. Certain types of glutathione S-transferase (GST), such as Pi class GST (referred to as GSTP1), are more highly expressed in a wide variety of human cancer tissues compared to their corresponding normal tissues. Pi class GST is thus a cancer cell molecular marker and potential target for overcoming resistance to chemotherapy. Here, we report that 4-bromo-1,8-naphthalimide (BrNaph) is a practical fluorogenic GST substrate. We have found that HE-BrNaph, an N-hydroxyethyl derivative, shows remarkable fluorescence enhancement upon GST-catalyzed S
Identifiants
pubmed: 31357346
pii: S0039-9140(19)30684-8
doi: 10.1016/j.talanta.2019.06.059
pii:
doi:
Substances chimiques
Fluorescent Dyes
0
Naphthalimides
0
GSTP1 protein, human
EC 2.5.1.18
Glutathione S-Transferase pi
EC 2.5.1.18
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
633-640Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.