Controlling magnesium corrosion and degradation-regulating mineralization using matrix GLA protein.
Animals
Calcium-Binding Proteins
/ chemistry
Collagen
/ chemistry
Corrosion
Extracellular Matrix Proteins
/ chemistry
Green Fluorescent Proteins
/ metabolism
HEK293 Cells
Human Umbilical Vein Endothelial Cells
/ metabolism
Humans
Implants, Experimental
Magnesium
/ chemistry
Male
Metals
/ chemistry
Mice, Inbred BALB C
Mice, Nude
Minerals
/ chemistry
Rats
Spectroscopy, Fourier Transform Infrared
Surface Properties
Tissue Scaffolds
/ chemistry
X-Ray Microtomography
Matrix Gla Protein
Magnesium
Matrix GLA Protein (MGP)
Mineralization
Journal
Acta biomaterialia
ISSN: 1878-7568
Titre abrégé: Acta Biomater
Pays: England
ID NLM: 101233144
Informations de publication
Date de publication:
15 10 2019
15 10 2019
Historique:
received:
30
12
2018
revised:
10
05
2019
accepted:
19
05
2019
pubmed:
23
7
2019
medline:
9
9
2020
entrez:
23
7
2019
Statut:
ppublish
Résumé
Magnesium (Mg) alloys are embraced for their biodegradability and biocompatibility. However, Mg degrades spontaneously in the biological environment in vivo and in vitro, triggering deposition of calcium phosphate on the metal. Upon complete metal absorption, minerals remain in the tissue, which could lead to pathogenic calcification. Hence, our aims are to test the feasibility of matrix GLA protein (MGP) to locally inhibit Mg mineralization that is induced by metal alloy degradation. MGP is a small secretory protein that has been shown to inhibit soft tissue calcification. We exposed Mg to MGP, stably transfected into mammalian cells. Results showed that less calcium and phosphorous deposition on the Mg surface when MGP was present relative to when it was not. In the in vivo mouse intramuscular model conducted for 4 and 6 weeks, Mg rods were embedded in collagen scaffolds, seeded with cells overexpressing MGP. Microtomography, electron dispersive x-ray spectroscopy, and histology assessments revealed lower deposited mineral volume around Mg rods from the MGP group. Compared to other groups, higher volume loss after implantation was observed from the MGP group at both time points, indicating a higher corrosion rate without the protective mineral layer. This study is the first to our knowledge to demonstrate that local exposure to a biomolecule, such as MGP, can modulate the corrosion of Mg-based implants. These findings may have important implications for the future design of endovascular stents and orthopedic devices.
Identifiants
pubmed: 31330328
pii: S1742-7061(19)30375-7
doi: 10.1016/j.actbio.2019.05.048
pii:
doi:
Substances chimiques
Calcium-Binding Proteins
0
Extracellular Matrix Proteins
0
Metals
0
Minerals
0
Green Fluorescent Proteins
147336-22-9
Collagen
9007-34-5
Magnesium
I38ZP9992A
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
142-151Subventions
Organisme : NIH HHS
ID : S10 OD021533
Pays : United States
Informations de copyright
Copyright © 2019 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.