Symptomatic and asymptomatic cases of African swine fever in Tanzania.
African Swine Fever
/ epidemiology
African Swine Fever Virus
/ genetics
Animals
Asymptomatic Diseases
Base Sequence
DNA, Intergenic
Disease Outbreaks
Genome, Viral
Genotype
Phylogeny
Polymerase Chain Reaction
Sequence Analysis
Sequence Analysis, DNA
Sus scrofa
/ virology
Swine
Tanzania
/ epidemiology
African swine fever disease
Tanzania
asymptomatic
phylogenetic
symptomatic
Journal
Transboundary and emerging diseases
ISSN: 1865-1682
Titre abrégé: Transbound Emerg Dis
Pays: Germany
ID NLM: 101319538
Informations de publication
Date de publication:
Nov 2019
Nov 2019
Historique:
received:
19
03
2019
revised:
12
07
2019
accepted:
12
07
2019
pubmed:
22
7
2019
medline:
13
2
2020
entrez:
21
7
2019
Statut:
ppublish
Résumé
African swine fever (ASF) is an acute, highly contagious and deadly viral haemorrhagic disease of domestic pigs caused by African swine fever virus (ASFV). In ASF endemic countries, there are an increasing number of reports on circulating ASFV strains with different levels of virulence causing a broad range of clinical symptoms in susceptible animals. Tanzania, where ASFV is endemic since 2001, recorded several outbreaks including symptomatic and asymptomatic cases between 2015 and 2017. We collected 35 clinical samples from four outbreaks for diagnostic confirmation and sequenced the partial B646L (p72), the full E183L (p54) gene, the central variable region of the B602L gene and the intergenic region between the I73R and I329L genes to characterize molecularly the new ASFV isolates and analyse their relatedness with previously reported Tanzanian and foreign isolates. We detected ASFV in 21 samples, 15 from symptomatic and six from asymptomatic pigs. Phylogenetic analyses based on the partial p72 gene and the complete p54 (E183L) genes revealed that the ASFVs in samples from symptomatic pigs belonged to genotypes II and those in samples from asymptomatic pigs belonged to genotype IX. The CVR profiles of the p72 genotype II and genotype IX isolates differed between each other and from previously published Tanzanian sequences. The sequence analysis of the intergenic region between the I73R and I329L for the 2017 genotype II isolates showed the absence of one GGAATATATA motif in those isolates. This study showed the simultaneous circulation of two different ASFV genotypes with different levels of pathogenicity in Tanzania. Since the existence of sub-clinically infected pigs may contribute to the persistence of the virus, our findings suggest continuous surveillance and characterization of ASFV isolates in disease-endemic regions.
Substances chimiques
DNA, Intergenic
0
Banques de données
GENBANK
['GQ410770', 'GQ410769', 'KF706366', 'KF706367', 'KF706365', 'KF706364']
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2402-2410Subventions
Organisme : International Atomic Energy Agency (IAEA)
Organisme : Government of Tanzania through the Ministry of Livestock Development and Fisheries
Informations de copyright
© 2019 Blackwell Verlag GmbH.
Références
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